HUCK-MCF-Nikon N-SIM/N-STORM

This microscope consolidates two super resolution techniques on one microscope stand.
N-SIM (structured illumination microscopy)

Double the Resolution of Conventional Optical Microscopes (xy 115 nm, z 300 nm)
Applicable to any sample prepared for conventional fluorescence microscopy – no special fluorophore needed
Fast Temporal Resolution of Approx. 1 sec/frame
Specifications
Motorized inverted Nikon ECLIPSE Ti-E TIRF microscope with perfect focus system
Lasers: 405 nm, 488 nm, 561 nm, 640 nm
Objective lens:
CFI SR Apochromat TIRF 100×oil (NA1.49)
CFI 20x
ORCA-Flash 4.0 sCMOS camera
NIS-Elements Ar
For more information visit https://www.nikoninstruments.com/Products/Super-resolution/N-SIM-E-Super-Resolution


N-STORM (STochastic Optical Reconstruction Microscopy)

The N-STORM super-resolution microscope provides 10 times that of conventional optical microscopes
Lateral (XY) resolution ~20-30nm
Axial (Z) resolution ~50nm
For more information visit https://www.nikoninstruments.com/Products/Super-resolution/N-STORM-Super-Resolution

Fig. 1. Mitochondria in FluoCells® Prepared Slide #1 bovine pulmonary artery endothelial cells (BPAEC) imaged with wide-field (left) and SIM super resolution (right). It is clearly shown that one can obtain images at a higher resolution with SIM. Ex: 561 nm; Label: MitoTracker® Red CMXRos; Microscope: Nikon N-SIM/N-STORM Super Resolution (newly installed). Bar=500 nm.