Dr. Yandong Zhou has a long interest in unveiling the mystery of Ca2+ signaling, a combination of molecular biology, biochemistry, cell biology, structure biology and single-cell physiological approaches will be utilized to understand how the Ca2+ channels are controlled. Current research area includes:
1. The STIM and Orai proteins have become recognized as a major and ubiquitous means for generating Ca2+ signals and maintaining Ca2+ homeostasis in cells. Our previous studies have explored this area and provided insight into the activation process of STIM proteins within cells. Current research focuses on the mechanisms underlying STIM protein activation by Ca2+ store-sensing or temperature change, and the precise molecular coupling of STIM proteins to Orai channels, focusing on the highly distinct STIM and Orai isoforms, and utilizing precise molecular/genetic probes, imaging technology, and gene-deleted cell lines and animal models.
2. We also have interests in investigating the gating mechanism of Two pore channels (TPCs). The TPCs are channels that allow the flow of positively charged ions and are located on the membranes of endolysosomes. These channels are vital for intracellular calcium signaling, and permeable for Na+ ion under some specific condition, and play a significant role in a range of pathophysiological processes, including cell growth and development, metabolism, and the progression of cancer.
3. Focusing on how the STIM/Orai pathway can be modified to regulate the process of fibrosis, determining transcriptional changes related to STIM-specific Ca2+ signal generation, and utilizing a novel STIM-specific inhibitory peptide, Orai3-M4x, to regulate cell remodeling.