TY - JOUR
T1 - αchymotrypsin catalyzed hydrolysis of n-benzoyl-l-tyrosine ethyl ester anchored in βcyclodextrin
AU - Kumble, Sandeep Prabhu
AU - Ramadoss, Candadai S.
N1 - Funding Information:
We would like to thank Dr. P. R. Krishnaswamy for the encouragement. SPK also thanks the Council of Scientific and Industrial Research for the award of Junior Research Fellowship.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1995
Y1 - 1995
N2 - N-Benzoyl-L-tyrosine ethyl ester, a substrate for αchymotrypsin (EC. 3.4.21.1) was solubilised by βcyclodextrin at a molar ratio of 1.0:2.0 but not by αcyclodextrin. The completely water soluble BTEE-βcyclodextrin complex served as an excellent substrate for the enzyme αchymotrypsin. The rate of hydrolysis of such a cyclodextrin anchored BTEE was identical to that of the substrate in Triton X-100 micelles, but was higher than the methanol solubilised substrate. The pH-activity profile was similar for all the three substrate preparations. The specificity constant (kcat/Km) was higher in the case of BTEE-βcyclodextrin compared to the methanol and Triton X-100 solubilised substrates. However, the enthalpy of activation was found to be increased in the case of Triton X-100 and βcyclodextrin anchored substrates.
AB - N-Benzoyl-L-tyrosine ethyl ester, a substrate for αchymotrypsin (EC. 3.4.21.1) was solubilised by βcyclodextrin at a molar ratio of 1.0:2.0 but not by αcyclodextrin. The completely water soluble BTEE-βcyclodextrin complex served as an excellent substrate for the enzyme αchymotrypsin. The rate of hydrolysis of such a cyclodextrin anchored BTEE was identical to that of the substrate in Triton X-100 micelles, but was higher than the methanol solubilised substrate. The pH-activity profile was similar for all the three substrate preparations. The specificity constant (kcat/Km) was higher in the case of BTEE-βcyclodextrin compared to the methanol and Triton X-100 solubilised substrates. However, the enthalpy of activation was found to be increased in the case of Triton X-100 and βcyclodextrin anchored substrates.
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U2 - 10.3109/10242429509003190
DO - 10.3109/10242429509003190
M3 - Article
AN - SCOPUS:0028796208
SN - 1024-2422
VL - 12
SP - 281
EP - 291
JO - Biocatalysis and Biotransformation
JF - Biocatalysis and Biotransformation
IS - 4
ER -