Abstract
Objective: WIND (WOUND INDUCED DEDIFFERENTIATION) are important transcription factors belonging to ERF/AP2 (ETHYLENE RESPONSE FACTOR/ APETALA 2) family. The genes were first found to bind to ethylene response element GCC-BOX and dehydration response element DRE in Arabidopsis thaliana to respond to drought signals and regulate ethylene levels. Recent studies have found that WIND genes also play a key role in plant wound signal response, callus formation and adventitious shoot production. Previous studies have explored the mechanisms of the WIND gene controlling callus formation and adventitious shoot regeneration in Arabidopsis thaliana. However, the functions of WIND in woody plants remain unclear. The functions of WIND genes in wound signal response and adventitious shoot regeneration will be explored in Populus euphratica. A theoretical basis for solving the regeneration problem of P. euphratica at the molecular level will be provided. Methods: Gene cloning, qRT-PCR and transgenic phenotype analysis were used. Results: Two WIND genes of P. euphratica were cloned as PeWINDl and PeWIND2, respectively. The coding regions were 1 050 bp and 1 032 bp, encoding 349 and 343 amino acids, respectively. Subcellular localization analysis showed that both genes were functional in the nucleus. Gene expression analysis showed that PeWINDl and PeWIND2 expressed in roots, stems, leaves, and calli. The highest expression level was found in calli. The time course expression analysis showed that the expression levels of PeWINDl and PeWIND2 were first increased and then decreased within 24 h after wound stimulation, and peaked at 1 h after wound stimulation. Phenotypic statistics of transgenic plants showed that the regeneration ability of adventitious shoots was enhanced after overexpression of PeWINDl and PeWIND2. Conclusion; PeWINDl and PeWIND2 responded to wound signal and the expression levels of PeWINDl and PeWIND2 were first increased and then decreased within 24 h after wound stimulation. PeWINDl and PeWIND2 promoted adventitious shoot regeneration from stems of poplars.
Translated title of the contribution | Cloning and Functional Analysis of WINDs gene in Populus euphratica |
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Original language | Chinese (Traditional) |
Pages (from-to) | 9-20 |
Number of pages | 12 |
Journal | China Biotechnology |
Volume | 42 |
Issue number | 10 |
DOIs | |
State | Published - 2022 |
All Science Journal Classification (ASJC) codes
- Accounting
- Business, Management and Accounting (miscellaneous)
- Development
- Economics, Econometrics and Finance (miscellaneous)