TY - JOUR
T1 - A 2D surface morphology-composition gradient panel for protein-binding assays
AU - Wei, Lai
AU - Vogler, Erwin A.
AU - Ritty, Timothy M.
AU - Lakhtakia, Akhlesh
N1 - Funding Information:
LW is grateful for a Materials Research Institute Graduate Fellowship . AL and TMR acknowledge a seed grant from the Woodward Research Foundation .
PY - 2011/12/1
Y1 - 2011/12/1
N2 - We designed a two-dimensional (2D) morphology-composition gradient panel, which has a gradient in surface morphology at constant chemical composition along its length and a gradient in surface chemical composition without change of surface morphology in the orthogonal direction. The panels, which exhibit a 2D gradient in surface wettability, were fabricated by thermolysis of parylene-C followed by oxygen-plasma treatment for various times. As a demonstration study, binding of three different biotinylated serum proteins (B-IgG, B-A, and B-TF) to the 2D panel was investigated. The 2D gradient panels will facilitate development of optimized binding surfaces for various biotechnological applications.
AB - We designed a two-dimensional (2D) morphology-composition gradient panel, which has a gradient in surface morphology at constant chemical composition along its length and a gradient in surface chemical composition without change of surface morphology in the orthogonal direction. The panels, which exhibit a 2D gradient in surface wettability, were fabricated by thermolysis of parylene-C followed by oxygen-plasma treatment for various times. As a demonstration study, binding of three different biotinylated serum proteins (B-IgG, B-A, and B-TF) to the 2D panel was investigated. The 2D gradient panels will facilitate development of optimized binding surfaces for various biotechnological applications.
UR - http://www.scopus.com/inward/record.url?scp=80054706282&partnerID=8YFLogxK
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U2 - 10.1016/j.msec.2011.09.001
DO - 10.1016/j.msec.2011.09.001
M3 - Article
AN - SCOPUS:80054706282
SN - 0928-4931
VL - 31
SP - 1861
EP - 1866
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 8
ER -