A calorimetric biosensing system for clinical diagnostic applications

David E. Gaddes, Srinivas A. Tadigadapa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

In this paper we report a calorimetrie sensing system for the detection of clinically relevant biomolecules. The calorimetrie biosensor consists of a thin (∼20 μm) micromachined Y-cut quartz crystal resonator (QCR) placed in close proximity to a fluidic chamber packed with an immobilized enzyme in alginate beads. This configuration enables a detection mechanism physically separated from the solution of interest and is thereby free from fouling. The performance of this biosensor is demonstrated by detection of urea, creatinine and hydrogen peroxide in phosphate buffer via a flow injection analysis (FIA) technique. This work demonstrates a sensing modality in which the sensor itself is not fouled or contaminated by the solution of interest. Such a system enables reuse and reliability for long term sampling measurements. Further, the immobilized enzyme is easily discarded and replaced with a new reaction column. We envision a biosensing system which can perform rapid measurements to detect biomarkers such as glucose, creatinine, urea and lactate in urine and blood continuously over extended periods of time.

Original languageEnglish (US)
Title of host publication2015 IEEE SENSORS - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479982028
DOIs
StatePublished - Dec 31 2015
Event14th IEEE SENSORS - Busan, Korea, Republic of
Duration: Nov 1 2015Nov 4 2015

Publication series

Name2015 IEEE SENSORS - Proceedings

Other

Other14th IEEE SENSORS
Country/TerritoryKorea, Republic of
CityBusan
Period11/1/1511/4/15

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electronic, Optical and Magnetic Materials
  • Spectroscopy
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'A calorimetric biosensing system for clinical diagnostic applications'. Together they form a unique fingerprint.

Cite this