TY - JOUR
T1 - A Cell Phone-Based Microphotometric System for Rapid Antimicrobial Susceptibility Testing
AU - Kadlec, Meichei Wang
AU - You, David
AU - Liao, Joseph C.
AU - Wong, Pak Kin
N1 - Funding Information:
The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the NIH Health Director’s New Innovator Award (1DP2OD007161-01) and NIAID (1U01AI082457-01 and 2R44AI088756-03).
PY - 2014/6
Y1 - 2014/6
N2 - This study demonstrates a low-cost, portable diagnostic system for rapid antimicrobial susceptibility testing in resource-limited settings. To determine the antimicrobial resistance phenotypically, the growth of pathogens in microwell arrays is detected under different antibiotic conditions. The use of a colorimetric cell viability reagent is shown to significantly improve the sensitivity of the assay compared with standard absorbance spectroscopy. Gas-permeable microwell arrays are incorporated for facilitating rapid bacterial growth and eliminating the requirement of bulky supporting equipment. Antibiotics can also be precoated in the microwell array to simplify the assay protocol toward point-of-care applications. Furthermore, a low-cost cell phone-based microphotometric system is developed for detecting the bacterial growth in the microwell array. By optimizing the operating conditions, the system allows antimicrobial susceptibility testing for samples with initial concentrations from 101 to 106 cfu/mL. Using urinary tract infection as the model system, we demonstrate rapid antimicrobial resistance profiling for uropathogens in both culture media and urine. With its simplicity and cost-effectiveness, the cell phone-based microphotometric system is anticipated to have broad applicability in resource-limited settings toward the management of infectious diseases caused by multidrug-resistant pathogens.
AB - This study demonstrates a low-cost, portable diagnostic system for rapid antimicrobial susceptibility testing in resource-limited settings. To determine the antimicrobial resistance phenotypically, the growth of pathogens in microwell arrays is detected under different antibiotic conditions. The use of a colorimetric cell viability reagent is shown to significantly improve the sensitivity of the assay compared with standard absorbance spectroscopy. Gas-permeable microwell arrays are incorporated for facilitating rapid bacterial growth and eliminating the requirement of bulky supporting equipment. Antibiotics can also be precoated in the microwell array to simplify the assay protocol toward point-of-care applications. Furthermore, a low-cost cell phone-based microphotometric system is developed for detecting the bacterial growth in the microwell array. By optimizing the operating conditions, the system allows antimicrobial susceptibility testing for samples with initial concentrations from 101 to 106 cfu/mL. Using urinary tract infection as the model system, we demonstrate rapid antimicrobial resistance profiling for uropathogens in both culture media and urine. With its simplicity and cost-effectiveness, the cell phone-based microphotometric system is anticipated to have broad applicability in resource-limited settings toward the management of infectious diseases caused by multidrug-resistant pathogens.
UR - http://www.scopus.com/inward/record.url?scp=84901058136&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901058136&partnerID=8YFLogxK
U2 - 10.1177/2211068213491095
DO - 10.1177/2211068213491095
M3 - Article
C2 - 23697894
AN - SCOPUS:84901058136
SN - 2211-0682
VL - 19
SP - 258
EP - 266
JO - Journal of Laboratory Automation
JF - Journal of Laboratory Automation
IS - 3
ER -