A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC

Hridayabhiranjan Shukla; Gerald A. Gillespie; Rakesh Srivastava; Francis Collins; Michael J. Chorney

doi:10.1016/0888-7543(91)90178-H

A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC

Hridayabhiranjan Shukla, Gerald A. Gillespie, Rakesh Srivastava, Francis Collins, Michael J. Chorney

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Abstract

By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Qa-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Qa-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.

Original language	English (US)
Pages (from-to)	905-914
Number of pages	10
Journal	Genomics
Volume	10
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(91)90178-H
State	Published - Aug 1991

All Science Journal Classification (ASJC) codes

Genetics

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10.1016/0888-7543(91)90178-H

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@article{1ae249792ae84dd49ab0e5e48a6dea51,

title = "A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC",

abstract = "By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Qa-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Qa-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.",

author = "Hridayabhiranjan Shukla and Gillespie, {Gerald A.} and Rakesh Srivastava and Francis Collins and Chorney, {Michael J.}",

note = "Funding Information: The authors thank Dr. Sherman Weissman for his generous support and helpful discussions during the performance of this research. This work was supported in part by a grant from the Children{\textquoteright}s Miracle Network Telethon Fund (M.J.C.) and NIH Grants CA47519 and AI26334-OlA2 (R.S.).",

year = "1991",

month = aug,

doi = "10.1016/0888-7543(91)90178-H",

language = "English (US)",

volume = "10",

pages = "905--914",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "4",

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T1 - A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC

AU - Shukla, Hridayabhiranjan

AU - Gillespie, Gerald A.

AU - Srivastava, Rakesh

AU - Collins, Francis

AU - Chorney, Michael J.

N1 - Funding Information: The authors thank Dr. Sherman Weissman for his generous support and helpful discussions during the performance of this research. This work was supported in part by a grant from the Children’s Miracle Network Telethon Fund (M.J.C.) and NIH Grants CA47519 and AI26334-OlA2 (R.S.).

PY - 1991/8

Y1 - 1991/8

N2 - By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Qa-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Qa-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.

AB - By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Qa-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Qa-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.

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JO - Genomics

JF - Genomics

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ER -

A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC

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