TY - JOUR
T1 - A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC
AU - Shukla, Hridayabhiranjan
AU - Gillespie, Gerald A.
AU - Srivastava, Rakesh
AU - Collins, Francis
AU - Chorney, Michael J.
N1 - Funding Information:
The authors thank Dr. Sherman Weissman for his generous support and helpful discussions during the performance of this research. This work was supported in part by a grant from the Children’s Miracle Network Telethon Fund (M.J.C.) and NIH Grants CA47519 and AI26334-OlA2 (R.S.).
PY - 1991/8
Y1 - 1991/8
N2 - By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Qa-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Qa-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.
AB - By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Qa-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Qa-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.
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U2 - 10.1016/0888-7543(91)90178-H
DO - 10.1016/0888-7543(91)90178-H
M3 - Article
C2 - 1916822
AN - SCOPUS:0025785214
SN - 0888-7543
VL - 10
SP - 905
EP - 914
JO - Genomics
JF - Genomics
IS - 4
ER -