TY - JOUR
T1 - A complement factor B-like cDNA clone from the zebrafish (Brachydanio rerio)
AU - Seeger, Angela
AU - Mayer, Werner E.
AU - Klein, Jan
PY - 1996/4
Y1 - 1996/4
N2 - An important molecule in the activation of the complement system in vertebrates is factor B, a serine protease with a molecular mass of 95,000. Factor B and the complement component C2 are thought to have arisen by gene duplication. In mammals and in Xenopus the factor B gene is linked to the major histocompatibility complex (MHC), whereas in domestic fowl it segregates independently of the MHC. Here we describe the isolation of a cDNA clone coding for factor B in the zebrafish, Brachydanio rerio. The deduced protein sequence exhibits a characteristic mosaic structure consisting of the short consensus repeat (SCR), the von Willebrand factor, and the serine protease domains. The estimated time of factor B and C2 divergence (approximately 350 million years ago), combined with the fact that C2 has thus far been found only in mammals, suggest that the factor B-C2 gene duplication occurred after the divergence of mammal-like reptiles from other reptiles and hence also birds. After the duplication, the C2 component evolved significantly faster than factor B.
AB - An important molecule in the activation of the complement system in vertebrates is factor B, a serine protease with a molecular mass of 95,000. Factor B and the complement component C2 are thought to have arisen by gene duplication. In mammals and in Xenopus the factor B gene is linked to the major histocompatibility complex (MHC), whereas in domestic fowl it segregates independently of the MHC. Here we describe the isolation of a cDNA clone coding for factor B in the zebrafish, Brachydanio rerio. The deduced protein sequence exhibits a characteristic mosaic structure consisting of the short consensus repeat (SCR), the von Willebrand factor, and the serine protease domains. The estimated time of factor B and C2 divergence (approximately 350 million years ago), combined with the fact that C2 has thus far been found only in mammals, suggest that the factor B-C2 gene duplication occurred after the divergence of mammal-like reptiles from other reptiles and hence also birds. After the duplication, the C2 component evolved significantly faster than factor B.
UR - http://www.scopus.com/inward/record.url?scp=0030130850&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030130850&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(96)00002-8
DO - 10.1016/0161-5890(96)00002-8
M3 - Article
C2 - 8700167
AN - SCOPUS:0030130850
SN - 0161-5890
VL - 33
SP - 511
EP - 520
JO - Molecular Immunology
JF - Molecular Immunology
IS - 6
ER -