TY - JOUR
T1 - A computerized microspectrophotometer using fibre optics for transmission and detection of light
AU - Neely, John
AU - Townend, William J.
AU - Combs, John W.
PY - 1984/3
Y1 - 1984/3
N2 - A computerized microspectrophotometer was developed to provide rapid and accurate sequential measurements on cells, including nuclear DNA content, by fluorescence staining, photometric grain counting from autoradiographs, and nuclear size estimated by measurement aperture area. To alleviate bulk and vibrations caused by additional stepper motors, the excitation light and filters as well as the emission filters and photomultipliers were detached from the main microscope frame. The light was directed to and from the microscope through fibre optic bundles. This configuration provided an excellent light gathering faculty and made optical alignment easier. The machine provided highly accurate sequential measurements on a relatively large number of cells by static photometry. 1984 Blackwell Science Ltd
AB - A computerized microspectrophotometer was developed to provide rapid and accurate sequential measurements on cells, including nuclear DNA content, by fluorescence staining, photometric grain counting from autoradiographs, and nuclear size estimated by measurement aperture area. To alleviate bulk and vibrations caused by additional stepper motors, the excitation light and filters as well as the emission filters and photomultipliers were detached from the main microscope frame. The light was directed to and from the microscope through fibre optic bundles. This configuration provided an excellent light gathering faculty and made optical alignment easier. The machine provided highly accurate sequential measurements on a relatively large number of cells by static photometry. 1984 Blackwell Science Ltd
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U2 - 10.1111/j.1365-2818.1984.tb00496.x
DO - 10.1111/j.1365-2818.1984.tb00496.x
M3 - Article
C2 - 6716463
AN - SCOPUS:84985224983
SN - 0022-2720
VL - 133
SP - 313
EP - 322
JO - Journal of Microscopy
JF - Journal of Microscopy
IS - 3
ER -