A computerized microspectrophotometer using fibre optics for transmission and detection of light

John Neely; William J. Townend; John W. Combs

doi:10.1111/j.1365-2818.1984.tb00496.x

A computerized microspectrophotometer using fibre optics for transmission and detection of light

John Neely
, William J. Townend
, John W. Combs

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A computerized microspectrophotometer was developed to provide rapid and accurate sequential measurements on cells, including nuclear DNA content, by fluorescence staining, photometric grain counting from autoradiographs, and nuclear size estimated by measurement aperture area. To alleviate bulk and vibrations caused by additional stepper motors, the excitation light and filters as well as the emission filters and photomultipliers were detached from the main microscope frame. The light was directed to and from the microscope through fibre optic bundles. This configuration provided an excellent light gathering faculty and made optical alignment easier. The machine provided highly accurate sequential measurements on a relatively large number of cells by static photometry. 1984 Blackwell Science Ltd

Original language	English (US)
Pages (from-to)	313-322
Number of pages	10
Journal	Journal of Microscopy
Volume	133
Issue number	3
DOIs	https://doi.org/10.1111/j.1365-2818.1984.tb00496.x
State	Published - Mar 1984

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine
Histology

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10.1111/j.1365-2818.1984.tb00496.x

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title = "A computerized microspectrophotometer using fibre optics for transmission and detection of light",

abstract = "A computerized microspectrophotometer was developed to provide rapid and accurate sequential measurements on cells, including nuclear DNA content, by fluorescence staining, photometric grain counting from autoradiographs, and nuclear size estimated by measurement aperture area. To alleviate bulk and vibrations caused by additional stepper motors, the excitation light and filters as well as the emission filters and photomultipliers were detached from the main microscope frame. The light was directed to and from the microscope through fibre optic bundles. This configuration provided an excellent light gathering faculty and made optical alignment easier. The machine provided highly accurate sequential measurements on a relatively large number of cells by static photometry. 1984 Blackwell Science Ltd",

author = "John Neely and Townend, \{William J.\} and Combs, \{John W.\}",

year = "1984",

month = mar,

doi = "10.1111/j.1365-2818.1984.tb00496.x",

language = "English (US)",

volume = "133",

pages = "313--322",

journal = "Journal of Microscopy",

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AU - Neely, John

AU - Townend, William J.

AU - Combs, John W.

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N2 - A computerized microspectrophotometer was developed to provide rapid and accurate sequential measurements on cells, including nuclear DNA content, by fluorescence staining, photometric grain counting from autoradiographs, and nuclear size estimated by measurement aperture area. To alleviate bulk and vibrations caused by additional stepper motors, the excitation light and filters as well as the emission filters and photomultipliers were detached from the main microscope frame. The light was directed to and from the microscope through fibre optic bundles. This configuration provided an excellent light gathering faculty and made optical alignment easier. The machine provided highly accurate sequential measurements on a relatively large number of cells by static photometry. 1984 Blackwell Science Ltd

AB - A computerized microspectrophotometer was developed to provide rapid and accurate sequential measurements on cells, including nuclear DNA content, by fluorescence staining, photometric grain counting from autoradiographs, and nuclear size estimated by measurement aperture area. To alleviate bulk and vibrations caused by additional stepper motors, the excitation light and filters as well as the emission filters and photomultipliers were detached from the main microscope frame. The light was directed to and from the microscope through fibre optic bundles. This configuration provided an excellent light gathering faculty and made optical alignment easier. The machine provided highly accurate sequential measurements on a relatively large number of cells by static photometry. 1984 Blackwell Science Ltd

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ER -

A computerized microspectrophotometer using fibre optics for transmission and detection of light

Abstract

All Science Journal Classification (ASJC) codes

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