A General Strategy to Control Viscosity Sensitivity of Molecular Rotor-Based Fluorophores

Songtao Ye, Han Zhang, Jinyu Fei, Charles H. Wolstenholme, Xin Zhang

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


Molecular rotor-based fluorophores (RBFs) have been widely used in many fields. However, the lack of control of their viscosity sensitivity limits their application. Herein, this problem is resolved by chemically installing extended π-rich alternating carbon-carbon linkages between the rotational electron donors and acceptors of RBFs. The data reveal that the length of the linkage strongly influences the viscosity sensitivity, likely resulting from varying height of the energy barriers between the fluorescent planar and the dark twisted configurations. Three RBF derivatives that span a wide range of viscosity sensitivities were designed. These RBFs demonstrated, through a dual-color imaging strategy, that they can differentiate misfolded protein oligomers and insoluble aggregates, both in test tubes and live cells. Beyond RBFs, it is envisioned that this chemical mechanism might be generally applicable to a wide range of photoisomerizable and aggregation-induced emission fluorophores.

Original languageEnglish (US)
Pages (from-to)1339-1346
Number of pages8
JournalAngewandte Chemie - International Edition
Issue number3
StatePublished - Jan 18 2021

All Science Journal Classification (ASJC) codes

  • Catalysis
  • General Chemistry


Dive into the research topics of 'A General Strategy to Control Viscosity Sensitivity of Molecular Rotor-Based Fluorophores'. Together they form a unique fingerprint.

Cite this