TY - JOUR
T1 - A genome survey of Moniliophthora perniciosa gives new insights into Witches' Broom Disease of cacao
AU - Mondego, Jorge M.C.
AU - Carazzolle, Marcelo F.
AU - Costa, Gustavo G.L.
AU - Formighieri, Eduardo F.
AU - Parizzi, Lucas P.
AU - Rincones, Johana
AU - Cotomacci, Carolina
AU - Carraro, Dirce M.
AU - Cunha, Anderson F.
AU - Carrer, Helaine
AU - Vidal, Ramon O.
AU - Estrela, Raíssa C.
AU - García, Odalys
AU - Thomazella, Daniela P.T.
AU - de Oliveira, Bruno V.
AU - Pires, Acássia B.L.
AU - Rio, Maria Carolina
AU - Araújo, Marcos Renato R.
AU - de Moraes, Marcos H.
AU - Castro, Luis A.B.
AU - Gramacho, Karina P.
AU - Gonçalves, Marilda S.
AU - Neto, José P.Moura
AU - Neto, Aristóteles Góes
AU - Barbosa, Luciana V.
AU - Guiltinan, Mark J.
AU - Bailey, Bryan A.
AU - Meinhardt, Lyndel W.
AU - Cascardo, Julio C.M.
AU - Pereira, Gonçalo A.G.
N1 - Funding Information:
We thank CENAPAD-SP (Centro Nacional de Processamento de Alto Desempenho de São Paulo) for data processing support, all sequencing technician for excellent technical assistance, especially Robson Dias (UESC – Brazil), Sulamita Freitas Franco (UNICAMP – Brazil) and Vitor Hugo de Almeida e Silva (UNICAMP – Brazil), and Dr. Francisco Javier Medrano for review and commentaries about the article. We are indebted to CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Projects 68.0032/2001-0 and 42.2279/2006-8), SEAGRI (Secretaria de Agri-cultura, Irrigação e Reforma Agrária do Estado da Bahia), CARGILL/FUN-CAMP/UNICAMP convenium (number C. 3007), FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) and CAPES (Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior) for financial support.
PY - 2008
Y1 - 2008
N2 - Background: The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD) in cacao (Theobroma cacao). It is a hemibiotrophic pathogen that colonizes the apoplast of cacao's meristematic tissues as a biotrophic pathogen, switching to a saprotrophic lifestyle during later stages of infection. M. perniciosa, together with the related species M. roreri, are pathogens of aerial parts of the plant, an uncommon characteristic in the order Agaricales. A genome survey (1.9× coverage) of M. perniciosa was analyzed to evaluate the overall gene content of this phytopathogen. Results: Genes encoding proteins involved in retrotransposition, reactive oxygen species (ROS) resistance, drug efflux transport and cell wall degradation were identified. The great number of genes encoding cytochrome P450 monooxygenases (1.15% of gene models) indicates that M. perniciosa has a great potential for detoxification, production of toxins and hormones; which may confer a high adaptive ability to the fungus. We have also discovered new genes encoding putative secreted polypeptides rich in cysteine, as well as genes related to methylotrophy and plant hormone biosynthesis (gibberellin and auxin). Analysis of gene families indicated that M. perniciosa have similar amounts of carboxylesterases and repertoires of plant cell wall degrading enzymes as other hemibiotrophic fungi. In addition, an approach for normalization of gene family data using incomplete genome data was developed and applied in M. perniciosa genome survey. Conclusion: This genome survey gives an overview of the M. perniciosa genome, and reveals that a significant portion is involved in stress adaptation and plant necrosis, two necessary characteristics for a hemibiotrophic fungus to fulfill its infection cycle. Our analysis provides new evidence revealing potential adaptive traits that may play major roles in the mechanisms of pathogenicity in the M. perniciosa/ cacao pathosystem.
AB - Background: The basidiomycete fungus Moniliophthora perniciosa is the causal agent of Witches' Broom Disease (WBD) in cacao (Theobroma cacao). It is a hemibiotrophic pathogen that colonizes the apoplast of cacao's meristematic tissues as a biotrophic pathogen, switching to a saprotrophic lifestyle during later stages of infection. M. perniciosa, together with the related species M. roreri, are pathogens of aerial parts of the plant, an uncommon characteristic in the order Agaricales. A genome survey (1.9× coverage) of M. perniciosa was analyzed to evaluate the overall gene content of this phytopathogen. Results: Genes encoding proteins involved in retrotransposition, reactive oxygen species (ROS) resistance, drug efflux transport and cell wall degradation were identified. The great number of genes encoding cytochrome P450 monooxygenases (1.15% of gene models) indicates that M. perniciosa has a great potential for detoxification, production of toxins and hormones; which may confer a high adaptive ability to the fungus. We have also discovered new genes encoding putative secreted polypeptides rich in cysteine, as well as genes related to methylotrophy and plant hormone biosynthesis (gibberellin and auxin). Analysis of gene families indicated that M. perniciosa have similar amounts of carboxylesterases and repertoires of plant cell wall degrading enzymes as other hemibiotrophic fungi. In addition, an approach for normalization of gene family data using incomplete genome data was developed and applied in M. perniciosa genome survey. Conclusion: This genome survey gives an overview of the M. perniciosa genome, and reveals that a significant portion is involved in stress adaptation and plant necrosis, two necessary characteristics for a hemibiotrophic fungus to fulfill its infection cycle. Our analysis provides new evidence revealing potential adaptive traits that may play major roles in the mechanisms of pathogenicity in the M. perniciosa/ cacao pathosystem.
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U2 - 10.1186/1471-2164-9-548
DO - 10.1186/1471-2164-9-548
M3 - Article
C2 - 19019209
AN - SCOPUS:60849102140
SN - 1471-2164
VL - 9
JO - BMC genomics
JF - BMC genomics
M1 - 548
ER -