A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice

Fred R. Sattler; Stephen Paolucci; John W. Kreider; Roger L. Ladda

doi:10.1016/0277-5379(82)90010-4

A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice

Fred R. Sattler, Stephen Paolucci, John W. Kreider, Roger L. Ladda

Department of Pediatrics

Research output: Contribution to journal › Article › peer-review

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Abstract

PLC/PRF/5 is a human liver cancer cell line which synthesizes hepatitis B virus surface antigen (HBsAg). These cells produced tumor in 6 of 8 (75%) congenitally athymic nude mice at 13 of 28 subcutaneous injection sites and in 13 of 14 (93%) mice inoculated intraperitoneally. Tumors were successfully transplanted to 4 of 6 additional nude mice. Tumor growth was rapid. Growth of cephalad tumors was significantly greater than for caudal tumors (0.39 mm/day versus 0.28 mm/day). Microscopic examination of tumors showed moderately well-differentiated hepatocellular carcinoma. Foci of identical cells were present in pulmonary veins in 7 of 14 tumor-bearing animals. Tumor cell karyotype was identical with that of PLC/PRF/5 cells. In addition, HBsAg was detectable in high titer in animals with extensive tumor. Biological features of PLC/PRF/5-induced tumors in nude mice appeared to closely resemble human hepatocellular carcinoma. Moreover, HBsAg may provide a marker of tumor growth.

Original language	English (US)
Pages (from-to)	381-389
Number of pages	9
Journal	European Journal of Cancer and Clinical Oncology
Volume	18
Issue number	4
DOIs	https://doi.org/10.1016/0277-5379(82)90010-4
State	Published - Apr 1982

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Oncology

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title = "A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice",

abstract = "PLC/PRF/5 is a human liver cancer cell line which synthesizes hepatitis B virus surface antigen (HBsAg). These cells produced tumor in 6 of 8 (75%) congenitally athymic nude mice at 13 of 28 subcutaneous injection sites and in 13 of 14 (93%) mice inoculated intraperitoneally. Tumors were successfully transplanted to 4 of 6 additional nude mice. Tumor growth was rapid. Growth of cephalad tumors was significantly greater than for caudal tumors (0.39 mm/day versus 0.28 mm/day). Microscopic examination of tumors showed moderately well-differentiated hepatocellular carcinoma. Foci of identical cells were present in pulmonary veins in 7 of 14 tumor-bearing animals. Tumor cell karyotype was identical with that of PLC/PRF/5 cells. In addition, HBsAg was detectable in high titer in animals with extensive tumor. Biological features of PLC/PRF/5-induced tumors in nude mice appeared to closely resemble human hepatocellular carcinoma. Moreover, HBsAg may provide a marker of tumor growth.",

author = "Sattler, {Fred R.} and Stephen Paolucci and Kreider, {John W.} and Ladda, {Roger L.}",

note = "Funding Information: *This work was supported by grants from the American Cancer Society Inc. (IN 109) and National Cancer Institute (Ca 18450). Address for reprint requests: Fred R. Sattler, M.D., 500 University Drive, Hershey, PA 17033, U.S.A.",

year = "1982",

month = apr,

doi = "10.1016/0277-5379(82)90010-4",

language = "English (US)",

volume = "18",

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journal = "European Journal of Cancer and Clinical Oncology",

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T1 - A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice

AU - Sattler, Fred R.

AU - Paolucci, Stephen

AU - Kreider, John W.

AU - Ladda, Roger L.

N1 - Funding Information: *This work was supported by grants from the American Cancer Society Inc. (IN 109) and National Cancer Institute (Ca 18450). Address for reprint requests: Fred R. Sattler, M.D., 500 University Drive, Hershey, PA 17033, U.S.A.

PY - 1982/4

Y1 - 1982/4

N2 - PLC/PRF/5 is a human liver cancer cell line which synthesizes hepatitis B virus surface antigen (HBsAg). These cells produced tumor in 6 of 8 (75%) congenitally athymic nude mice at 13 of 28 subcutaneous injection sites and in 13 of 14 (93%) mice inoculated intraperitoneally. Tumors were successfully transplanted to 4 of 6 additional nude mice. Tumor growth was rapid. Growth of cephalad tumors was significantly greater than for caudal tumors (0.39 mm/day versus 0.28 mm/day). Microscopic examination of tumors showed moderately well-differentiated hepatocellular carcinoma. Foci of identical cells were present in pulmonary veins in 7 of 14 tumor-bearing animals. Tumor cell karyotype was identical with that of PLC/PRF/5 cells. In addition, HBsAg was detectable in high titer in animals with extensive tumor. Biological features of PLC/PRF/5-induced tumors in nude mice appeared to closely resemble human hepatocellular carcinoma. Moreover, HBsAg may provide a marker of tumor growth.

AB - PLC/PRF/5 is a human liver cancer cell line which synthesizes hepatitis B virus surface antigen (HBsAg). These cells produced tumor in 6 of 8 (75%) congenitally athymic nude mice at 13 of 28 subcutaneous injection sites and in 13 of 14 (93%) mice inoculated intraperitoneally. Tumors were successfully transplanted to 4 of 6 additional nude mice. Tumor growth was rapid. Growth of cephalad tumors was significantly greater than for caudal tumors (0.39 mm/day versus 0.28 mm/day). Microscopic examination of tumors showed moderately well-differentiated hepatocellular carcinoma. Foci of identical cells were present in pulmonary veins in 7 of 14 tumor-bearing animals. Tumor cell karyotype was identical with that of PLC/PRF/5 cells. In addition, HBsAg was detectable in high titer in animals with extensive tumor. Biological features of PLC/PRF/5-induced tumors in nude mice appeared to closely resemble human hepatocellular carcinoma. Moreover, HBsAg may provide a marker of tumor growth.

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A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice

Abstract

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