TY - GEN
T1 - A hybrid electrokinetic bioprocessor for static and dynamic pathogen manipulation in physiological samples
AU - Gao, Jian
AU - Sin, Mandy L.Y.
AU - Wong, Pak Kin
PY - 2011
Y1 - 2011
N2 - This paper reports a hybrid electrokinetic bioprocessor for label-free manipulation of bacterial pathogens in physiological samples toward lab-on-a-chip applications. While most electrokinetic techniques only function effectively in low-conductivity buffers, hybrid electrokinetics enables operation in high-conductivity samples (∼1 S/m). We tackle the major technical hurdle of electrode instability using a Ti-Au-Ti sandwich configuration for manipulating high conductivity samples. The technique combines short-range electrophoresis and dielectrophoresis, and long-range AC electrothermal flow to dramatically improve its effectiveness. The device is the first AC electrokinetic static/dynamic concentrator that can work directly with physiological fluids, such as urine and buffy coats. We show that uropathogenic bacteria and biowarfare agents can be concentrated for over 3 orders of magnitude.
AB - This paper reports a hybrid electrokinetic bioprocessor for label-free manipulation of bacterial pathogens in physiological samples toward lab-on-a-chip applications. While most electrokinetic techniques only function effectively in low-conductivity buffers, hybrid electrokinetics enables operation in high-conductivity samples (∼1 S/m). We tackle the major technical hurdle of electrode instability using a Ti-Au-Ti sandwich configuration for manipulating high conductivity samples. The technique combines short-range electrophoresis and dielectrophoresis, and long-range AC electrothermal flow to dramatically improve its effectiveness. The device is the first AC electrokinetic static/dynamic concentrator that can work directly with physiological fluids, such as urine and buffy coats. We show that uropathogenic bacteria and biowarfare agents can be concentrated for over 3 orders of magnitude.
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U2 - 10.1109/TRANSDUCERS.2011.5969403
DO - 10.1109/TRANSDUCERS.2011.5969403
M3 - Conference contribution
AN - SCOPUS:80052125227
SN - 9781457701573
T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
SP - 270
EP - 273
BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Y2 - 5 June 2011 through 9 June 2011
ER -