TY - GEN
T1 - A hybrid electrokinetic processor for isolating exfoliated cancer cells and circulating tumor cells in physiological samples
AU - Gao, Jian
AU - Riahi, Reza
AU - Sin, Mandy L.Y.
AU - Wong, Pak Kin
PY - 2011
Y1 - 2011
N2 - This paper reports a hybrid electrokinetic processor that combines electrophoresis (EP), dielectrophoresis (DEP) and AC electrothermal flow (ACEF) for isolating exfoliated cancer cells (ECCs) and circulating tumor cells (CTCs) in physiological samples. While most electrokinetic techniques only function in low-conductivity buffers, hybrid electrokinetics enables effective operation in high-conductivity physiological fluids (∼ 1 S/m) such as blood, buffy coat and urine. Using a 3-electrode configuration with optimized AC voltage, frequency and DC offset, we demonstrate that breast cancer cells can be isolated in buffy coats and biological fluids in 10 sec.
AB - This paper reports a hybrid electrokinetic processor that combines electrophoresis (EP), dielectrophoresis (DEP) and AC electrothermal flow (ACEF) for isolating exfoliated cancer cells (ECCs) and circulating tumor cells (CTCs) in physiological samples. While most electrokinetic techniques only function in low-conductivity buffers, hybrid electrokinetics enables effective operation in high-conductivity physiological fluids (∼ 1 S/m) such as blood, buffy coat and urine. Using a 3-electrode configuration with optimized AC voltage, frequency and DC offset, we demonstrate that breast cancer cells can be isolated in buffy coats and biological fluids in 10 sec.
UR - https://www.scopus.com/pages/publications/84883757955
UR - https://www.scopus.com/inward/citedby.url?scp=84883757955&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883757955
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1472
EP - 1474
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -
