TY - JOUR
T1 - A kinase-dead version of FERONIA receptor-like kinase has dose-dependent impacts on rosette morphology and RALF1-mediated stomatal movements
AU - Chakravorty, David
AU - Yu, Yunqing
AU - Assmann, Sarah M.
N1 - Funding Information:
This research was supported by NSF-MCB grant 1715826 to S.M.A. and by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R01GM126079. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We thank Prof. Gabriele Monshausen (Pennsylvania State University) for providing seeds of the fer mutants and the FER and FERK565R transgenic lines. We thank Prof. Alice Cheung for fertile discussions on FER signaling mechanisms.
Publisher Copyright:
© 2018 Federation of European Biochemical Societies
PY - 2018/10
Y1 - 2018/10
N2 - The receptor-like kinase FERONIA (FER) pleiotropically affects plant reproduction, development, and stress tolerance. We recently showed that the FER ligand RALF1 promotes stomatal closure and inhibits stomatal opening in a G-protein-dependent manner. FER responses have been designated as kinase-dependent or kinase-independent, based largely on fer complementation assays employing a kinase-dead FERK565R. Our quantification of FERK565R-GFP transcript and FERK565R-GFP protein in fer complementation lines reveal that, even within individual complementation lines, different levels of FERK565R expression prevail. FERK565R-GFP expression comparable to that of FER in Col-0 plants fail to elicit complementation of either fer rosette phenotypes or RALF1-elicited stomatal movements, whereas overexpression levels of FERK565R-GFP result in complementation. These results suggest possible alternative interpretations of previous conclusions regarding kinase-independent FER signaling.
AB - The receptor-like kinase FERONIA (FER) pleiotropically affects plant reproduction, development, and stress tolerance. We recently showed that the FER ligand RALF1 promotes stomatal closure and inhibits stomatal opening in a G-protein-dependent manner. FER responses have been designated as kinase-dependent or kinase-independent, based largely on fer complementation assays employing a kinase-dead FERK565R. Our quantification of FERK565R-GFP transcript and FERK565R-GFP protein in fer complementation lines reveal that, even within individual complementation lines, different levels of FERK565R expression prevail. FERK565R-GFP expression comparable to that of FER in Col-0 plants fail to elicit complementation of either fer rosette phenotypes or RALF1-elicited stomatal movements, whereas overexpression levels of FERK565R-GFP result in complementation. These results suggest possible alternative interpretations of previous conclusions regarding kinase-independent FER signaling.
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U2 - 10.1002/1873-3468.13249
DO - 10.1002/1873-3468.13249
M3 - Article
C2 - 30207378
AN - SCOPUS:85053681543
SN - 0014-5793
VL - 592
SP - 3429
EP - 3437
JO - FEBS Letters
JF - FEBS Letters
IS - 20
ER -