A mass spectrometric solution to the address problem of combinatorial libraries

Christopher L. Brummel; Irene N.W. Lee; Ying Zhou; Stephen J. Benkovic; Nicholas Winograd

doi:10.1126/science.8153627

A mass spectrometric solution to the address problem of combinatorial libraries

Christopher L. Brummel, Irene N.W. Lee, Ying Zhou, Stephen J. Benkovic, Nicholas Winograd

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139 Scopus citations

Abstract

The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- to 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.

Original language	English (US)
Pages (from-to)	399-402
Number of pages	4
Journal	Science
Volume	264
Issue number	5157
DOIs	https://doi.org/10.1126/science.8153627
State	Published - 1994

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title = "A mass spectrometric solution to the address problem of combinatorial libraries",

abstract = "The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- to 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.",

author = "Brummel, {Christopher L.} and Lee, {Irene N.W.} and Ying Zhou and Benkovic, {Stephen J.} and Nicholas Winograd",

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T1 - A mass spectrometric solution to the address problem of combinatorial libraries

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AU - Lee, Irene N.W.

AU - Zhou, Ying

AU - Benkovic, Stephen J.

AU - Winograd, Nicholas

PY - 1994

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AB - The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- to 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.

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A mass spectrometric solution to the address problem of combinatorial libraries

Abstract

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