TY - GEN
T1 - A micro device for separation of erythrocytes and leukocytes in human blood
AU - Zheng, Siyang
AU - Tai, Yu Chong
AU - Kasdan, Harvey
PY - 2005/12/1
Y1 - 2005/12/1
N2 - We report a MEMS device for continuous and binary separation of leukocytes (white blood cells, WBCs) and erythrocytes (red blood cells, RBCs) by their sizes. The system consists of PDMS cylindrical pillars inside fluidic chamber on glass slides. A 420 μm lateral separation was achieved for 5 μm and 10 μm beads. The critical particle size for separation was successful predicted by simulation and experimentally determined by calibration with polystyrene beads. The separation function (lateral displacement versus particle size) was also measured experimentally. Diluted whole blood and blood fraction of concentrated leukocytes were tested with the devices with erythrocyte to leukocyte ratio in agreement with blood count results. Experiments with fluorescent labeled leukocytes confirm the separation of leukocytes from erythrocytes and measured the separation efficiency.
AB - We report a MEMS device for continuous and binary separation of leukocytes (white blood cells, WBCs) and erythrocytes (red blood cells, RBCs) by their sizes. The system consists of PDMS cylindrical pillars inside fluidic chamber on glass slides. A 420 μm lateral separation was achieved for 5 μm and 10 μm beads. The critical particle size for separation was successful predicted by simulation and experimentally determined by calibration with polystyrene beads. The separation function (lateral displacement versus particle size) was also measured experimentally. Diluted whole blood and blood fraction of concentrated leukocytes were tested with the devices with erythrocyte to leukocyte ratio in agreement with blood count results. Experiments with fluorescent labeled leukocytes confirm the separation of leukocytes from erythrocytes and measured the separation efficiency.
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M3 - Conference contribution
AN - SCOPUS:33846912743
SN - 0780387406
SN - 9780780387409
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 1024
EP - 1027
BT - Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
T2 - 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Y2 - 1 September 2005 through 4 September 2005
ER -