A microfluidic device for epigenomic profiling using 100 cells

Zhenning Cao, Changya Chen, Bing He, Kai Tan, Chang Lu

Research output: Contribution to journalArticlepeer-review

89 Scopus citations


The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many new enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.

Original languageEnglish (US)
Pages (from-to)959-962
Number of pages4
JournalNature methods
Issue number10
StatePublished - Sep 29 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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