A microfluidic device for epigenomic profiling using 100 cells

Zhenning Cao; Changya Chen; Bing He; Kai Tan; Chang Lu

doi:10.1038/nmeth.3488

A microfluidic device for epigenomic profiling using 100 cells

Zhenning Cao, Changya Chen, Bing He, Kai Tan, Chang Lu

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many new enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.

Original language	English (US)
Pages (from-to)	959-962
Number of pages	4
Journal	Nature methods
Volume	12
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.3488
State	Published - Sep 29 2015

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.3488

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A microfluidic device for epigenomic profiling using 100 cells

Abstract

All Science Journal Classification (ASJC) codes

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