@article{aa6f31aacb5e459e8eaef4b3f04363c5,
title = "A microfluidic device for epigenomic profiling using 100 cells",
abstract = "The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many new enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.",
author = "Zhenning Cao and Changya Chen and Bing He and Kai Tan and Chang Lu",
note = "Funding Information: We thank the Genomics Research Laboratory of Virginia Bioinformatics Institute and Genomics Division of Iowa Institute of Human Genetics for providing sequencing service. We thank L. Van Tol and University of Iowa Institute for Clinical and Translational Science for providing computing support. This work was supported by US National Institutes of Health grants EB017855 (K.T. and C.L.), CA174577 (C.L.), EB017235 (C.L.), HG006130 (K.T.) and GM104369 (K.T.) and a seed grant from Virginia Tech Institute for Critical Technology and Applied Science (C.L.). Publisher Copyright: {\textcopyright} 2015 Nature America, Inc. All rights reserved.",
year = "2015",
month = sep,
day = "29",
doi = "10.1038/nmeth.3488",
language = "English (US)",
volume = "12",
pages = "959--962",
journal = "Nature methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "10",
}