A model for validation of radioimmunoassay kit reagents: Measurement of follitropin and lutropin in blood and urine

S. J. Santner; R. J. Santen; H. E. Kulin; L. M. Demers

doi:10.1093/clinchem/27.11.1892

A model for validation of radioimmunoassay kit reagents: Measurement of follitropin and lutropin in blood and urine

S. J. Santner, R. J. Santen, H. E. Kulin, L. M. Demers

Department of Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

We measured lutropin and follitropin in blood and urine with radioimmunoassay kits from Diagnostic Products Corporation and compared the results with those obtained by use of reagents from the National Institutes of Health (NIH) and the World Health Organization (WHO). The urine standard (second IRP-HMG) from WHO, the blood standard (LER-907) from NIH, and the commercial standards all effected similar displacement of trace material when the commercial gonadotropin kit reagents were used. Highly significant correlations were achieved for these hormones in blood or urine on comparing commercial and NIH/WHO reagents. Serial dilutions of urine samples produced similar relative potencies with the commercial reagents. Conversion factors are presented to relate results for LER-907, second IRP, or commercial standards. Commercially available reagents can provide a practical and reliable means of gonadotropin radioimmunoassay in blood or urine.

Original language	English (US)
Pages (from-to)	1892-1895
Number of pages	4
Journal	Clinical chemistry
Volume	27
Issue number	11
DOIs	https://doi.org/10.1093/clinchem/27.11.1892
State	Published - 1981

All Science Journal Classification (ASJC) codes

Clinical Biochemistry
Biochemistry, medical

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10.1093/clinchem/27.11.1892

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title = "A model for validation of radioimmunoassay kit reagents: Measurement of follitropin and lutropin in blood and urine",

abstract = "We measured lutropin and follitropin in blood and urine with radioimmunoassay kits from Diagnostic Products Corporation and compared the results with those obtained by use of reagents from the National Institutes of Health (NIH) and the World Health Organization (WHO). The urine standard (second IRP-HMG) from WHO, the blood standard (LER-907) from NIH, and the commercial standards all effected similar displacement of trace material when the commercial gonadotropin kit reagents were used. Highly significant correlations were achieved for these hormones in blood or urine on comparing commercial and NIH/WHO reagents. Serial dilutions of urine samples produced similar relative potencies with the commercial reagents. Conversion factors are presented to relate results for LER-907, second IRP, or commercial standards. Commercially available reagents can provide a practical and reliable means of gonadotropin radioimmunoassay in blood or urine.",

author = "Santner, {S. J.} and Santen, {R. J.} and Kulin, {H. E.} and Demers, {L. M.}",

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AU - Demers, L. M.

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AB - We measured lutropin and follitropin in blood and urine with radioimmunoassay kits from Diagnostic Products Corporation and compared the results with those obtained by use of reagents from the National Institutes of Health (NIH) and the World Health Organization (WHO). The urine standard (second IRP-HMG) from WHO, the blood standard (LER-907) from NIH, and the commercial standards all effected similar displacement of trace material when the commercial gonadotropin kit reagents were used. Highly significant correlations were achieved for these hormones in blood or urine on comparing commercial and NIH/WHO reagents. Serial dilutions of urine samples produced similar relative potencies with the commercial reagents. Conversion factors are presented to relate results for LER-907, second IRP, or commercial standards. Commercially available reagents can provide a practical and reliable means of gonadotropin radioimmunoassay in blood or urine.

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A model for validation of radioimmunoassay kit reagents: Measurement of follitropin and lutropin in blood and urine

Abstract

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