A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease

Brian L. Gilmore, Carly E. Winton, Andrew C. Demmert, Justin R. Tanner, Sam Bowman, Vasilea Karageorge, Kaya Patel, Zhi Sheng, Deborah F. Kelly

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.

Original languageEnglish (US)
Article number14440
JournalScientific reports
Volume5
DOIs
StatePublished - Sep 23 2015

All Science Journal Classification (ASJC) codes

  • General

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