A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease

Brian L. Gilmore; Carly E. Winton; Andrew C. Demmert; Justin R. Tanner; Sam Bowman; Vasilea Karageorge; Kaya Patel; Zhi Sheng; Deborah F. Kelly

doi:10.1038/srep14440

A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease

Brian L. Gilmore
, Carly E. Winton
, Andrew C. Demmert
, Justin R. Tanner
, Sam Bowman
, Vasilea Karageorge
, Kaya Patel
, Zhi Sheng
, Deborah F. Kelly

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

12 Citations (SciVal)

Abstract

We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.

Original language	English (US)
Article number	14440
Journal	Scientific reports
Volume	5
DOIs	https://doi.org/10.1038/srep14440
State	Published - Sep 23 2015

All Science Journal Classification (ASJC) codes

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10.1038/srep14440

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title = "A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease",

abstract = "We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.",

author = "Gilmore, \{Brian L.\} and Winton, \{Carly E.\} and Demmert, \{Andrew C.\} and Tanner, \{Justin R.\} and Sam Bowman and Vasilea Karageorge and Kaya Patel and Zhi Sheng and Kelly, \{Deborah F.\}",

note = "Funding Information: This research is supported by funds from Virginia Tech, the Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech, the Commonwealth Health Research Board [2080914], the Concern Foundation [303872], and NCI/NIH [R01CA193578] to D.F.K. C.W. is funded through the ICTAS Doctoral Scholar{\textquoteright}s program at Virginia Tech and the Medical Research Scholar{\textquoteright}s program at the Virginia Tech Carilion Research Institute.",

year = "2015",

month = sep,

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doi = "10.1038/srep14440",

language = "English (US)",

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T1 - A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease

AU - Gilmore, Brian L.

AU - Winton, Carly E.

AU - Demmert, Andrew C.

AU - Tanner, Justin R.

AU - Bowman, Sam

AU - Karageorge, Vasilea

AU - Patel, Kaya

AU - Sheng, Zhi

AU - Kelly, Deborah F.

N1 - Funding Information: This research is supported by funds from Virginia Tech, the Institute for Critical Technology and Applied Science (ICTAS) at Virginia Tech, the Commonwealth Health Research Board [2080914], the Concern Foundation [303872], and NCI/NIH [R01CA193578] to D.F.K. C.W. is funded through the ICTAS Doctoral Scholar’s program at Virginia Tech and the Medical Research Scholar’s program at the Virginia Tech Carilion Research Institute.

PY - 2015/9/23

Y1 - 2015/9/23

N2 - We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.

AB - We present a new molecular toolkit to investigate protein assemblies natively formed in the context of human disease. The system employs tunable microchips that can be decorated with switchable adaptor molecules to select for target proteins of interest and analyze them using molecular microscopy. Implementing our new streamlined microchip approach, we could directly visualize BRCA1 gene regulatory complexes from patient-derived cancer cells for the first time.

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A Molecular Toolkit to Visualize Native Protein Assemblies in the Context of Human Disease

Abstract

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