A mouse model of intracerebral hemorrhage using autologous blood infusion

Michal A. Rynkowski, Grace H. Kim, Ricardo J. Komotar, Marc L. Otten, Andrew F. Ducruet, Brad E. Zacharia, Christopher P. Kellner, David K. Hahn, Maxwell B. Merkow, Matthew C. Garrett, Robert M. Starke, Byung Moon Cho, Sergei A. Sosunov, Sander E. Connolly

Research output: Contribution to journalArticlepeer-review

126 Scopus citations


The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

Original languageEnglish (US)
Pages (from-to)122-128
Number of pages7
JournalNature Protocols
Issue number1
StatePublished - Jan 2008

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology


Dive into the research topics of 'A mouse model of intracerebral hemorrhage using autologous blood infusion'. Together they form a unique fingerprint.

Cite this