A mouse model of intracerebral hemorrhage using autologous blood infusion

Michal A. Rynkowski; Grace H. Kim; Ricardo J. Komotar; Marc L. Otten; Andrew F. Ducruet; Brad E. Zacharia; Christopher P. Kellner; David K. Hahn; Maxwell B. Merkow; Matthew C. Garrett; Robert M. Starke; Byung Moon Cho; Sergei A. Sosunov; Sander E. Connolly

doi:10.1038/nprot.2007.513

A mouse model of intracerebral hemorrhage using autologous blood infusion

Michal A. Rynkowski, Grace H. Kim, Ricardo J. Komotar, Marc L. Otten, Andrew F. Ducruet, Brad E. Zacharia, Christopher P. Kellner, David K. Hahn, Maxwell B. Merkow, Matthew C. Garrett, Robert M. Starke, Byung Moon Cho, Sergei A. Sosunov, Sander E. Connolly

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118 Scopus citations

Abstract

The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

Original language	English (US)
Pages (from-to)	122-128
Number of pages	7
Journal	Nature Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1038/nprot.2007.513
State	Published - Jan 2008

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1038/nprot.2007.513

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title = "A mouse model of intracerebral hemorrhage using autologous blood infusion",

abstract = "The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.",

author = "Rynkowski, {Michal A.} and Kim, {Grace H.} and Komotar, {Ricardo J.} and Otten, {Marc L.} and Ducruet, {Andrew F.} and Zacharia, {Brad E.} and Kellner, {Christopher P.} and Hahn, {David K.} and Merkow, {Maxwell B.} and Garrett, {Matthew C.} and Starke, {Robert M.} and Cho, {Byung Moon} and Sosunov, {Sergei A.} and Connolly, {Sander E.}",

note = "Funding Information: ACKNOWLEDGMENTS Dr. M.A.R. was supported by The Kosciuszko Foundation Postdoctoral Research Fellowship.",

year = "2008",

month = jan,

doi = "10.1038/nprot.2007.513",

language = "English (US)",

volume = "3",

pages = "122--128",

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AU - Rynkowski, Michal A.

AU - Kim, Grace H.

AU - Komotar, Ricardo J.

AU - Otten, Marc L.

AU - Ducruet, Andrew F.

AU - Zacharia, Brad E.

AU - Kellner, Christopher P.

AU - Hahn, David K.

AU - Merkow, Maxwell B.

AU - Garrett, Matthew C.

AU - Starke, Robert M.

AU - Cho, Byung Moon

AU - Sosunov, Sergei A.

AU - Connolly, Sander E.

N1 - Funding Information: ACKNOWLEDGMENTS Dr. M.A.R. was supported by The Kosciuszko Foundation Postdoctoral Research Fellowship.

PY - 2008/1

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N2 - The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

AB - The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

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A mouse model of intracerebral hemorrhage using autologous blood infusion

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