TY - GEN
T1 - A multiwell microfluidic device for probing the effect of antibiotics on Mycobacterium migration
AU - Li, Hui
AU - Liu, Yiming
AU - Wong, Pak Kin
N1 - Funding Information:
medium. Analysis of the trajectories of individual bacteria revealed distinct migratory patterns under different conditions (see color traces in Fig. 2b). The migratory behaviors are summarized in Fig. 2c. These results support M. bacteremicum could collectively avoid fatal antibiotics regardless of nutrition availability. CONCLUSION In summary, we demonstrate a multiwell microfluidic device for probing bacterial response to antibiotics. Our data suggests M. bacteremicum can sense both nutrition and antibiotics, and is capable of decision making to avoid fatal factors. Further work is required to elucidate the signaling mechanism govern the decision-making process and to investigate the environmental influence of the bacterial response on the emergence of antibiotic resistance. ACKNOWLEDGEMENTS We acknowledge funding support from NIH NIAID. REFERENCES [1] Bos, Julia, et al. "Emergence of antibiotic resistance from multinucleated bacterial filaments." Proceedings of the National Academy of Sciences 112.1 (2015): 178-183. [2] Zhang, Qiucen, et al. "Acceleration of emergence of bacterial antibiotic resistance in connected microenvi-ronments." Science 333.6050 (2011): 1764-1767. [3] Kindrachuk, Kristen N., et al. "Involvement of an ATP-dependent protease, PA0779/AsrA, in inducing heat shock in response to tobramycin in Pseudomonas aeruginosa." Antimicrobial agents and chemotherapy 55.5 (2011): 1874-1882. [4] Nguyen, Dao, et al. "Active starvation responses mediate antibiotic tolerance in biofilms and nutrient-limited bacteria." Science 334.6058 (2011): 982-986. CONTACT * Pak Kin Wong; phone: +1-814-863-5267; [email protected]
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - This paper reports the design, fabrication and characterization of a multiwell microfluidic device for investigating the response of Mycobacterium to various environmental conditions. Our results reveal Mycobacterium is capable of avoiding fatal antibiotics, independent of the availability of nutrition, by modulating the migratory patterns, underscoring an environmental microbiology factor on the emergence of antibiotic resistance.
AB - This paper reports the design, fabrication and characterization of a multiwell microfluidic device for investigating the response of Mycobacterium to various environmental conditions. Our results reveal Mycobacterium is capable of avoiding fatal antibiotics, independent of the availability of nutrition, by modulating the migratory patterns, underscoring an environmental microbiology factor on the emergence of antibiotic resistance.
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M3 - Conference contribution
AN - SCOPUS:85079660767
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 834
EP - 835
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -