A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Masayuki Takahashi; Reyad A. Elbarbary; Aiko Nakashima; Mayumi Abe; Norihiro Watanabe; Miwako Narita; Masuhiro Takahashi; Masato Tamura; Tetsuo Yoshida; Masayuki Nashimoto

doi:10.1016/j.canlet.2012.10.016

A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Masayuki Takahashi, Reyad A. Elbarbary, Aiko Nakashima, Mayumi Abe, Norihiro Watanabe, Miwako Narita, Masuhiro Takahashi, Masato Tamura, Tetsuo Yoshida, Masayuki Nashimoto

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19 Scopus citations

Abstract

tRNase Z^L-utilizing efficacious gene silencing is a gene control technology, which is based on the property that tRNase Z^L can cleave any target RNA under the direction of an appropriate small guide RNA (sgRNA). To find therapeutic sgRNAs to cure hematological malignancies, we investigated behavior of heptamer-type sgRNA. We demonstrated that a heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents and that it can induce apoptosis of the leukemia cells. Mouse xenograft experiments showed that a median survival of the mh1(Bcl-2)-treated mice was longer than that of the control mice.

Original language	English (US)
Pages (from-to)	362-368
Number of pages	7
Journal	Cancer Letters
Volume	328
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2012.10.016
State	Published - Jan 28 2013

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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title = "A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells",

abstract = "tRNase ZL-utilizing efficacious gene silencing is a gene control technology, which is based on the property that tRNase ZL can cleave any target RNA under the direction of an appropriate small guide RNA (sgRNA). To find therapeutic sgRNAs to cure hematological malignancies, we investigated behavior of heptamer-type sgRNA. We demonstrated that a heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents and that it can induce apoptosis of the leukemia cells. Mouse xenograft experiments showed that a median survival of the mh1(Bcl-2)-treated mice was longer than that of the control mice.",

author = "Masayuki Takahashi and Elbarbary, {Reyad A.} and Aiko Nakashima and Mayumi Abe and Norihiro Watanabe and Miwako Narita and Masuhiro Takahashi and Masato Tamura and Tetsuo Yoshida and Masayuki Nashimoto",

note = "Funding Information: We thank Miharu Takeda for technical assistance. This work was supported by Adaptable and Seamless Technology Transfer Program through Target-driven R&D, Japan Science and Technology Agency. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ",

year = "2013",

month = jan,

day = "28",

doi = "10.1016/j.canlet.2012.10.016",

language = "English (US)",

volume = "328",

pages = "362--368",

journal = "Cancer Letters",

issn = "0304-3835",

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T1 - A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

AU - Takahashi, Masayuki

AU - Elbarbary, Reyad A.

AU - Nakashima, Aiko

AU - Abe, Mayumi

AU - Watanabe, Norihiro

AU - Narita, Miwako

AU - Takahashi, Masuhiro

AU - Tamura, Masato

AU - Yoshida, Tetsuo

AU - Nashimoto, Masayuki

N1 - Funding Information: We thank Miharu Takeda for technical assistance. This work was supported by Adaptable and Seamless Technology Transfer Program through Target-driven R&D, Japan Science and Technology Agency. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

PY - 2013/1/28

Y1 - 2013/1/28

N2 - tRNase ZL-utilizing efficacious gene silencing is a gene control technology, which is based on the property that tRNase ZL can cleave any target RNA under the direction of an appropriate small guide RNA (sgRNA). To find therapeutic sgRNAs to cure hematological malignancies, we investigated behavior of heptamer-type sgRNA. We demonstrated that a heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents and that it can induce apoptosis of the leukemia cells. Mouse xenograft experiments showed that a median survival of the mh1(Bcl-2)-treated mice was longer than that of the control mice.

AB - tRNase ZL-utilizing efficacious gene silencing is a gene control technology, which is based on the property that tRNase ZL can cleave any target RNA under the direction of an appropriate small guide RNA (sgRNA). To find therapeutic sgRNAs to cure hematological malignancies, we investigated behavior of heptamer-type sgRNA. We demonstrated that a heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents and that it can induce apoptosis of the leukemia cells. Mouse xenograft experiments showed that a median survival of the mh1(Bcl-2)-treated mice was longer than that of the control mice.

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EP - 368

JO - Cancer Letters

JF - Cancer Letters

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A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Abstract

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