@article{cd7abf3d2aa7467e98008cb2ff3ace56,
title = "A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior",
abstract = "DREADDs are chemogenetic tools widely used to remotely control cellular signaling, neuronal activity, and behavior. Here we used a structure-based approach to develop a new Gi-coupled DREADD using the kappa-opioid receptor as a template (KORD) that is activated by the pharmacologically inert ligand salvinorin B (SALB). Activation of virally expressed KORD in several neuronal contexts robustly attenuated neuronal activity and modified behaviors. Additionally, co-expression of the KORD and the Gq-coupled M3-DREADD within the same neuronal population facilitated the sequential and bidirectional remote control of behavior. The availability of DREADDs activated by different ligands provides enhanced opportunities for investigating diverse physiological systems using multiplexed chemogenetic actuators.",
author = "Eyal Vardy and Robinson, {J. Elliott} and Chia Li and Olsen, {Reid H.J.} and DiBerto, {Jeffrey F.} and Giguere, {Patrick M.} and Sassano, {Flori M.} and Huang, {Xi Ping} and Hu Zhu and Urban, {Daniel J.} and White, {Kate L.} and Rittiner, {Joseph E.} and Crowley, {Nicole A.} and Pleil, {Kristen E.} and Mazzone, {Christopher M.} and Mosier, {Philip D.} and Juan Song and Kash, {Thomas L.} and Malanga, {C. J.} and Krashes, {Michael J.} and Roth, {Bryan L.}",
note = "Funding Information: This work was supported, in whole or in part, by the NIH BRAIN Initiative Grant UO1MH105892 (B.L.R. and T.L.K.) and by R01 DA017204 (B.L.R.), PO1DA035764 (B.L.R.), and the NIH-NIMH Psychoactive Drug Screening Program (B.L.R.); the Intramural Research Program of the NIH, The National Institute of Diabetes and Digestive and Kidney Diseases – NIDDK; DK075087, DK075089 (M.J.K.); and by R01AA019454, R00AA017668, NARSAD Young Investigator Award, and U01AA020911 (T.L.K.); and F31AA02228001 (N.A.C.), R01 AA018335 (C.J.M.), and F30 AA021312 (J.E.R.). Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",
year = "2015",
month = may,
day = "20",
doi = "10.1016/j.neuron.2015.03.065",
language = "English (US)",
volume = "86",
pages = "936--946",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",
}