Abstract
We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) real-time UV dose (fluence) determination; and 2) simple actinometric determination of a reactor factor that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This reactor factor replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k = 0.1471 cm2/mJ (with 95% confidence bounds of 0.1426 to 0.1516).
Original language | English (US) |
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Pages (from-to) | 674-680 |
Number of pages | 7 |
Journal | Journal of Photochemistry and Photobiology B: Biology |
Volume | 162 |
DOIs | |
State | Published - Sep 1 2016 |
All Science Journal Classification (ASJC) codes
- Radiation
- Radiological and Ultrasound Technology
- Biophysics
- Radiology Nuclear Medicine and imaging