TY - JOUR
T1 - A novel FRET-based fluorescent chemosensor of β-cyclodextrin derivative for TNT detection in aqueous solution
AU - Feng, Lijuan
AU - Tong, Cuiyan
AU - He, Yao
AU - Liu, Bingxin
AU - Wang, Chunyu
AU - Sha, Jie
AU - Lü, Changli
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China ( 21074019 ) and Natural Science Foundation of Jilin Province ( 20101539 ).
PY - 2014
Y1 - 2014
N2 - A new fluorescent sensor based on single water-soluble β-cyclodextrin (β-CD) molecule as the support carrier of donor and acceptor complex was designed, and used for TNT detection by fluorescence resonance energy transfer (FRET). In this sensing platform, per-6-amino-β-CD (per-6-NH 2-β-CD) was used as the detection vehicle. The probe dye of fluorescein 5(6)-isothiocyanate (FITC) was covalently linked onto the per-6-NH2-β-CD rim with the grafting molar ratio of 1:1, the residual amino groups of per-6-NH2-β-CD can adsorb TNT molecules by forming Meisenheimer complex (TNT-amine complex) with TNT. The absorbtion spectrum of this complex has a spectral overlapping with the emission of FITC in aqueous solution, so it can strongly suppress the fluorescence emission of the adjacent FITC through FRET on β-CD vehicle. This FRET-based fluorescent sensing technique provides a facile, ultrasensitive and selective detection method for TNT molecule. The observed linear fluorescence intensity change could allow the quantitative detection TNT with the detection limit of 20 nM in water.
AB - A new fluorescent sensor based on single water-soluble β-cyclodextrin (β-CD) molecule as the support carrier of donor and acceptor complex was designed, and used for TNT detection by fluorescence resonance energy transfer (FRET). In this sensing platform, per-6-amino-β-CD (per-6-NH 2-β-CD) was used as the detection vehicle. The probe dye of fluorescein 5(6)-isothiocyanate (FITC) was covalently linked onto the per-6-NH2-β-CD rim with the grafting molar ratio of 1:1, the residual amino groups of per-6-NH2-β-CD can adsorb TNT molecules by forming Meisenheimer complex (TNT-amine complex) with TNT. The absorbtion spectrum of this complex has a spectral overlapping with the emission of FITC in aqueous solution, so it can strongly suppress the fluorescence emission of the adjacent FITC through FRET on β-CD vehicle. This FRET-based fluorescent sensing technique provides a facile, ultrasensitive and selective detection method for TNT molecule. The observed linear fluorescence intensity change could allow the quantitative detection TNT with the detection limit of 20 nM in water.
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U2 - 10.1016/j.jlumin.2013.10.039
DO - 10.1016/j.jlumin.2013.10.039
M3 - Article
AN - SCOPUS:84887743836
SN - 0022-2313
VL - 146
SP - 502
EP - 507
JO - Journal of Luminescence
JF - Journal of Luminescence
ER -