Abstract
A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3- kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3- kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE! escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.
Original language | English (US) |
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Pages (from-to) | 135-138 |
Number of pages | 4 |
Journal | Genomics |
Volume | 31 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1996 |
All Science Journal Classification (ASJC) codes
- Genetics