A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status

Michael P. Coleman; Helen J. Ambrose; Laura Carrel; Andrea H. Németh; Huntington F. Willard; Kay E. Davies

doi:10.1006/geno.1996.0022

A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status

Michael P. Coleman
, Helen J. Ambrose
, Laura Carrel
, Andrea H. Németh
, Huntington F. Willard
, Kay E. Davies

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3- kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3- kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE! escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.

Original language	English (US)
Pages (from-to)	135-138
Number of pages	4
Journal	Genomics
Volume	31
Issue number	1
DOIs	https://doi.org/10.1006/geno.1996.0022
State	Published - Jan 1 1996

All Science Journal Classification (ASJC) codes

Genetics

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10.1006/geno.1996.0022

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title = "A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status",

abstract = "A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3- kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3- kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE! escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.",

author = "Coleman, \{Michael P.\} and Ambrose, \{Helen J.\} and Laura Carrel and N{\'e}meth, \{Andrea H.\} and Willard, \{Huntington F.\} and Davies, \{Kay E.\}",

note = "Funding Information: We are grateful to Dr. Derek Blake for helpful discussion and human fetal brain cDNA, Dr. George Dixon for human fetal brain mRNA, Drs. David Bentley and Jill Holland for the human cosmid library, and Dr. Chris Ward for helpful advice. We thank Steve Phelps and Tracey Flint for preparation of DNA from somatic cell hybrids. This work was funded by the Human Genome Mapping Project (M.P.C.), the Medical Research Council (H.A., A.N., and K.E.D.), and NIH (H.F.W. and L.C.).",

year = "1996",

month = jan,

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doi = "10.1006/geno.1996.0022",

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AU - Németh, Andrea H.

AU - Willard, Huntington F.

AU - Davies, Kay E.

N1 - Funding Information: We are grateful to Dr. Derek Blake for helpful discussion and human fetal brain cDNA, Dr. George Dixon for human fetal brain mRNA, Drs. David Bentley and Jill Holland for the human cosmid library, and Dr. Chris Ward for helpful advice. We thank Steve Phelps and Tracey Flint for preparation of DNA from somatic cell hybrids. This work was funded by the Human Genome Mapping Project (M.P.C.), the Medical Research Council (H.A., A.N., and K.E.D.), and NIH (H.F.W. and L.C.).

PY - 1996/1/1

Y1 - 1996/1/1

N2 - A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3- kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3- kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE! escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.

AB - A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3- kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3- kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE! escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status.

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A novel gene, DXS8237E, lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status

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