A Novel Heterobifunctional Linker for Formyl to Thiol Coupling

Ned D. Heindel; Huiru Zhao; Roger A. Egolf; Chien Hsing Chang; Keith J. Schray; Jacqueline G. Emrich; Joanne P. McLaughlin; David V. Woo

doi:10.1021/bc00012a008

A Novel Heterobifunctional Linker for Formyl to Thiol Coupling

Ned D. Heindel, Huiru Zhao, Roger A. Egolf, Chien Hsing Chang, Keith J. Schray, Jacqueline G. Emrich, Joanne P. McLaughlin, David V. Woo

Penn State Lehigh Valley

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A maleimide hydrazide has been synthesized as a heterobifunctional cross-linking agent for thiol to formyl coupling. This linker has been applied to the coupling of the monoclonal antibody 17–1A, or an Fab′ derived therefrom, to polyaldehyde dextran onto which the antineoplastic agent ellipticine has been attached. High binding avidities for the unshed antigen on the SW1116 colorectal tumor cell are retained in these drug-dextran-linker-antibody conjugates.

Original language	English (US)
Pages (from-to)	427-430
Number of pages	4
Journal	Bioconjugate Chemistry
Volume	2
Issue number	6
DOIs	https://doi.org/10.1021/bc00012a008
State	Published - May 1 1991

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/bc00012a008

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title = "A Novel Heterobifunctional Linker for Formyl to Thiol Coupling",

abstract = "A maleimide hydrazide has been synthesized as a heterobifunctional cross-linking agent for thiol to formyl coupling. This linker has been applied to the coupling of the monoclonal antibody 17–1A, or an Fab′ derived therefrom, to polyaldehyde dextran onto which the antineoplastic agent ellipticine has been attached. High binding avidities for the unshed antigen on the SW1116 colorectal tumor cell are retained in these drug-dextran-linker-antibody conjugates.",

author = "Heindel, {Ned D.} and Huiru Zhao and Egolf, {Roger A.} and Chang, {Chien Hsing} and Schray, {Keith J.} and Emrich, {Jacqueline G.} and McLaughlin, {Joanne P.} and Woo, {David V.}",

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AU - Heindel, Ned D.

AU - Zhao, Huiru

AU - Egolf, Roger A.

AU - Chang, Chien Hsing

AU - Schray, Keith J.

AU - Emrich, Jacqueline G.

AU - McLaughlin, Joanne P.

AU - Woo, David V.

PY - 1991/5/1

Y1 - 1991/5/1

N2 - A maleimide hydrazide has been synthesized as a heterobifunctional cross-linking agent for thiol to formyl coupling. This linker has been applied to the coupling of the monoclonal antibody 17–1A, or an Fab′ derived therefrom, to polyaldehyde dextran onto which the antineoplastic agent ellipticine has been attached. High binding avidities for the unshed antigen on the SW1116 colorectal tumor cell are retained in these drug-dextran-linker-antibody conjugates.

AB - A maleimide hydrazide has been synthesized as a heterobifunctional cross-linking agent for thiol to formyl coupling. This linker has been applied to the coupling of the monoclonal antibody 17–1A, or an Fab′ derived therefrom, to polyaldehyde dextran onto which the antineoplastic agent ellipticine has been attached. High binding avidities for the unshed antigen on the SW1116 colorectal tumor cell are retained in these drug-dextran-linker-antibody conjugates.

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JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

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ER -

A Novel Heterobifunctional Linker for Formyl to Thiol Coupling

Abstract

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