A novel, low temperature method for the preparation of ß-TCP/HAP biphasic nanostructured ceramic scaffold from natural cancellous bone

In this study, a novel method was used to synthesize ß-TCP/HAP biphasic ceramic scaffold from natural cancellous bone. Bovine bone was calcined to remove the organic content. The remaining material was then soaked in P₂O₅ solution for different periods. P ions were doped into cancellous bone and reacted with hydroxyapatite (HAP) to produce ß-tricalcium phosphate (ß-TCP) at room temperature without any subsequent high-temperature heat treatment. By increasing the soaking time, the crystalline phase composition of the calcined bone gradually changed from HAP into a HAP/ß-TCP biphasic structure with different HAP/TCP ratios. The crystallite size of HAP and ß-TCP was smaller than 100 nm.