A novel multiplex PCR method for detecting the major clonal complexes of MRSA in nasal isolates from a Pennsylvania hospital

Nathan D. Schwalm; Bindhu Verghese; Stephen J. Knabel

doi:10.1016/j.mimet.2011.05.024

A novel multiplex PCR method for detecting the major clonal complexes of MRSA in nasal isolates from a Pennsylvania hospital

Nathan D. Schwalm
, Bindhu Verghese
, Stephen J. Knabel

Food Science

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A novel multiplex PCR was developed which targeted virulence genes associated with the major clonal complexes (CCs) of healthcare- and community-associated methicillin-resistant Staphylococcus aureus (MRSA) in the USA. Most isolates (40/66) were identified as CC 5, while remaining isolates represented CCs 1, 8, 30, 45, 59, 133, and five isolates were not S. aureus.

Original language	English (US)
Pages (from-to)	379-382
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	86
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2011.05.024
State	Published - Sep 2011

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2011.05.024

Cite this

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abstract = "A novel multiplex PCR was developed which targeted virulence genes associated with the major clonal complexes (CCs) of healthcare- and community-associated methicillin-resistant Staphylococcus aureus (MRSA) in the USA. Most isolates (40/66) were identified as CC 5, while remaining isolates represented CCs 1, 8, 30, 45, 59, 133, and five isolates were not S. aureus.",

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AB - A novel multiplex PCR was developed which targeted virulence genes associated with the major clonal complexes (CCs) of healthcare- and community-associated methicillin-resistant Staphylococcus aureus (MRSA) in the USA. Most isolates (40/66) were identified as CC 5, while remaining isolates represented CCs 1, 8, 30, 45, 59, 133, and five isolates were not S. aureus.

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A novel multiplex PCR method for detecting the major clonal complexes of MRSA in nasal isolates from a Pennsylvania hospital

Abstract

All Science Journal Classification (ASJC) codes

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