TY - JOUR
T1 - A physical map of the Chinese chestnut (Castanea mollissima) genome and its integration with the genetic map
AU - Fang, Guang Chen
AU - Blackmon, Barbara P.
AU - Staton, Margaret E.
AU - Nelson, C. Dana
AU - Kubisiak, Thomas L.
AU - Olukolu, Bode A.
AU - Henry, David
AU - Zhebentyayeva, Tatyana
AU - Saski, Christopher A.
AU - Cheng, Chun Huai
AU - Monsanto, Megan
AU - Ficklin, Stephen
AU - Atkins, Michael
AU - Georgi, Laura L.
AU - Barakat, Abdelali
AU - Wheeler, Nicholas
AU - Carlson, John E.
AU - Sederoff, Ronald
AU - Abbott, Albert G.
N1 - Funding Information:
Acknowledgments This study was supported by the Plant Genome Research Program (PGRP) from the US National Science Foundation (NSF-PGRP 0605135) awarded to RRS. The Forest Health Initiative (FHI) awarded to AGA supported part of the map integration and comparative analyses. Plants used in this research were obtained from Greg Miller at the Empire Chestnut Company. The authors thank Dr. Paul Sisco for early support through The American Chestnut Foundation (TACF) and for markers in the earliest stages of this project and Dr. Bill Powell and Kathleen Baier for full-length cDNA sequence and in-depth discussion.
PY - 2013/4
Y1 - 2013/4
N2 - Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was used to assemble 126,445 BAC clones into 1,377 contigs and 12,919 singletons. Integration of the dense Chinese chestnut genetic map with the physical map was achieved via high-throughput hybridization using overgo probes derived from sequence-based genetic markers. A total of 1,026 probes were anchored to the physical map including 831 probes corresponding to 878 expressed sequence tag-based markers. Within the physical map, three BAC contigs were anchored to the three major fungal blight-resistant quantitative trait loci on chestnut linkage groups B, F, and G. A subset of probes corresponding to orthologous genes in poplar showed only a limited amount of conserved gene order between the poplar and chestnut genomes. The integrated genetic and physical map of Chinese chestnut is available at www. fagaceae. org/physical_maps.
AB - Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was used to assemble 126,445 BAC clones into 1,377 contigs and 12,919 singletons. Integration of the dense Chinese chestnut genetic map with the physical map was achieved via high-throughput hybridization using overgo probes derived from sequence-based genetic markers. A total of 1,026 probes were anchored to the physical map including 831 probes corresponding to 878 expressed sequence tag-based markers. Within the physical map, three BAC contigs were anchored to the three major fungal blight-resistant quantitative trait loci on chestnut linkage groups B, F, and G. A subset of probes corresponding to orthologous genes in poplar showed only a limited amount of conserved gene order between the poplar and chestnut genomes. The integrated genetic and physical map of Chinese chestnut is available at www. fagaceae. org/physical_maps.
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U2 - 10.1007/s11295-012-0576-6
DO - 10.1007/s11295-012-0576-6
M3 - Article
AN - SCOPUS:84875229854
SN - 1614-2942
VL - 9
SP - 525
EP - 537
JO - Tree Genetics and Genomes
JF - Tree Genetics and Genomes
IS - 2
ER -