A portable microfluidic paper-based device for elisa

X. Y. Liu; C. M. Cheng; A. W. Martinez; K. A. Mirica; X. J. Li; S. T. Phillips; M. Mascareñas; G. M. Whitesides

doi:10.1109/MEMSYS.2011.5734365

A portable microfluidic paper-based device for elisa

X. Y. Liu
, C. M. Cheng
, A. W. Martinez
, K. A. Mirica
, X. J. Li
, S. T. Phillips
, M. Mascareñas
, G. M. Whitesides

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

67 Scopus citations

Abstract

This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.

Original language	English (US)
Title of host publication	2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011
Pages	75-78
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2011.5734365
State	Published - Apr 13 2011
Event	24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 - Cancun, Mexico Duration: Jan 23 2011 → Jan 27 2011

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011
Country/Territory	Mexico
City	Cancun
Period	1/23/11 → 1/27/11

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2011.5734365

Cite this

Liu, X. Y., Cheng, C. M., Martinez, A. W., Mirica, K. A., Li, X. J., Phillips, S. T., Mascareñas, M., & Whitesides, G. M. (2011). A portable microfluidic paper-based device for elisa. In 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011 (pp. 75-78). Article 5734365 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2011.5734365

@inproceedings{c3ccd301d45a4e2ea8dbbe581d5e8315,

title = "A portable microfluidic paper-based device for elisa",

abstract = "This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.",

author = "Liu, \{X. Y.\} and Cheng, \{C. M.\} and Martinez, \{A. W.\} and Mirica, \{K. A.\} and Li, \{X. J.\} and Phillips, \{S. T.\} and M. Mascare{\~n}as and Whitesides, \{G. M.\}",

year = "2011",

month = apr,

day = "13",

doi = "10.1109/MEMSYS.2011.5734365",

language = "English (US)",

isbn = "9781424496327",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "75--78",

booktitle = "2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011",

note = "24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011 ; Conference date: 23-01-2011 Through 27-01-2011",

}

Liu, XY, Cheng, CM, Martinez, AW, Mirica, KA, Li, XJ, Phillips, ST, Mascareñas, M & Whitesides, GM 2011, A portable microfluidic paper-based device for elisa. in 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011., 5734365, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 75-78, 24th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2011, Cancun, Mexico, 1/23/11. https://doi.org/10.1109/MEMSYS.2011.5734365

A portable microfluidic paper-based device for elisa. / Liu, X. Y.; Cheng, C. M.; Martinez, A. W. et al.
2011 IEEE 24th International Conference on Micro Electro Mechanical Systems, MEMS 2011. 2011. p. 75-78 5734365 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

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AU - Cheng, C. M.

AU - Martinez, A. W.

AU - Mirica, K. A.

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AU - Phillips, S. T.

AU - Mascareñas, M.

AU - Whitesides, G. M.

PY - 2011/4/13

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N2 - This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.

AB - This paper describes a portable, three-dimensional (3D) microfluidic paper-based analytical device (3D-μPAD) for performing enzyme-linked immunosorbent assays (ELISA). To run an ELISA, the device requires only the addition of a small drop (2 μL per test zone) of sample and six drops (75 μL in total volume) of buffer solution. All the reagents required for the analysis are stored in dry form within the device, and are dissolved in the buffer and delivered to the test zones during the assay. The innovative feature of the device, which enables the delivery of different reagents and washes to the test zones without cross contamination, is a movable strip containing the test zones that can be moved manually through the device, and stops only at specified points where the test zones come into contact with different fluidic paths that sequentially transfer (i.e., wash) the reagents into the test zones. Using rabbit IgG as a model analyte, we performed an ELISA in 43 minutes, with a detection limit of 330 pM. We also demonstrated the detection of hepatitis B surface antigen (HBsAg) in serum.

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Y2 - 23 January 2011 through 27 January 2011

ER -

A portable microfluidic paper-based device for elisa

Abstract

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UN SDGs

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