@inbook{b3817114cf704584b06181674d030f24,
title = "A practical guide to the use of monolayer purification and affinity grids",
abstract = "Lipid monolayers have traditionally been used in electron microscopy (EM) to form two-dimensional (2D) protein arrays for structural studies by electron crystallography. More recently, monolayers containing Nickel-nitrilotriacetic acid (Ni-NTA) lipids have been used to combine the purification and preparation of single-particle EM specimens of His-tagged proteins into a single, convenient step. This monolayer purification technique was further simplified by introducing the Affinity Grid, an EM grid that features a predeposited Ni-NTA lipid-containing monolayer. In this contribution, we provide a detailed description for the use of monolayer purification and Affinity Grids, discuss their advantages and limitations, and present examples to illustrate specific applications of the methods.",
author = "Kelly, {Deborah F.} and Danijela Dukovski and Thomas Walz",
note = "Funding Information: Work on monolayer purification and Affinity Grids in the Walz laboratory is supported by NIH grant P01 GM062580 (to Stephen C. Harrison). TW is an investigator in the Howard Hughes Medical Institute.",
year = "2010",
doi = "10.1016/S0076-6879(10)81004-3",
language = "English (US)",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
number = "C",
pages = "83--107",
booktitle = "Methods in Enzymology",
address = "United States",
edition = "C",
}