TY - JOUR
T1 - A proteomic analysis of IVF follicular fluid in women ≤32 years old
AU - Estes, Stephanie J.
AU - Ye, Bin
AU - Qiu, Weiliang
AU - Cramer, Daniel
AU - Hornstein, Mark D.
AU - Missmer, Stacey A.
N1 - Funding Information:
Supported by National Institutes of Health grant no. HD32153 and the research fund of the Department of Obstetrics and Gynecology, Brigham and Women's Hospital.
PY - 2009/11
Y1 - 2009/11
N2 - Objective: To address the lack of predictors of IVF success by using proteomic biometrics. Design: Experimental study of follicular fluid specimens from a prospective cohort of IVF patients. Setting: Academic research laboratory and IVF program. Patient(s): Women ≤32 years old with <11 oocytes retrieved and no pregnancy were matched to women who had ≥11 oocytes and live birth (10 pairs). Year of cycle start and IVF down-regulation protocol were also matched. Intervention(s): Follicular fluid was separated by two-dimensional polyacrylamide gel electrophoresis followed by Sypro Ruby staining and comparison with PDQuest software. Logistic regression was incorporated to calculate the likelihood of live birth in relation to the protein spot of interest. Main Outcome Measure(s): Protein markers. Result(s): Liquid chromatography-tandem mass spectrometry and searching of sequence databases revealed 11 potential protein candidates. Haptoglobin alpha, predominantly fetal expressed T1 domain, mitochondrial integrity genome (ATPase), apolipoprotein H (beta-2 glycoprotein I), dihydrolipoyl dehydrogenase, lyzozyme C, fibrinogen alpha-chain, and immunoglobulin heavy chain V-III (region BRO) were found to have increased expression in the live birth group, whereas antithrombin, vitamin D-binding protein, and complement 3 were decreased. An ELISA confirmed a significantly lower level of antithrombin. Conclusion(s): Proteomic evaluation of follicular fluid is able to identify potential biomarkers of good versus poor responders in matched pairs of IVF patients.
AB - Objective: To address the lack of predictors of IVF success by using proteomic biometrics. Design: Experimental study of follicular fluid specimens from a prospective cohort of IVF patients. Setting: Academic research laboratory and IVF program. Patient(s): Women ≤32 years old with <11 oocytes retrieved and no pregnancy were matched to women who had ≥11 oocytes and live birth (10 pairs). Year of cycle start and IVF down-regulation protocol were also matched. Intervention(s): Follicular fluid was separated by two-dimensional polyacrylamide gel electrophoresis followed by Sypro Ruby staining and comparison with PDQuest software. Logistic regression was incorporated to calculate the likelihood of live birth in relation to the protein spot of interest. Main Outcome Measure(s): Protein markers. Result(s): Liquid chromatography-tandem mass spectrometry and searching of sequence databases revealed 11 potential protein candidates. Haptoglobin alpha, predominantly fetal expressed T1 domain, mitochondrial integrity genome (ATPase), apolipoprotein H (beta-2 glycoprotein I), dihydrolipoyl dehydrogenase, lyzozyme C, fibrinogen alpha-chain, and immunoglobulin heavy chain V-III (region BRO) were found to have increased expression in the live birth group, whereas antithrombin, vitamin D-binding protein, and complement 3 were decreased. An ELISA confirmed a significantly lower level of antithrombin. Conclusion(s): Proteomic evaluation of follicular fluid is able to identify potential biomarkers of good versus poor responders in matched pairs of IVF patients.
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U2 - 10.1016/j.fertnstert.2008.08.120
DO - 10.1016/j.fertnstert.2008.08.120
M3 - Article
C2 - 18980758
AN - SCOPUS:70350573022
SN - 0015-0282
VL - 92
SP - 1569
EP - 1578
JO - Fertility and sterility
JF - Fertility and sterility
IS - 5
ER -