TY - JOUR
T1 - A quantitative diagnostic method for oral mucous precancerosis by Rose Bengal fluorescence spectroscopy
AU - Zhang, Lei
AU - Bi, Liangjia
AU - Shi, Jinna
AU - Zhang, Zhiguo
AU - Cao, Wenwu
AU - Lin, Jiang
AU - Li, Chengzhang
AU - Bi, Jiarui
AU - Yu, Yang
N1 - Funding Information:
Acknowledgments This research was supported by the scientific research fund from the Department of Education, Heilongjiang Province (No: 11541148) and the research fund from the First Affiliated Hospital of Harbin Medical University (2011BS006).
PY - 2013/1
Y1 - 2013/1
N2 - A novel in vivo fluorescence spectroscopic diagnostic method has been developed in an animal model to make a quantified precancer diagnosis. In the study, 40 golden hamsters were randomly divided into four groups (groups A, B, C, and D), with group A being the control group and the other three groups being inducted at different precancer stages. A 1% Rose Bengal (RB) solution was used for the fluorescence spectroscopic diagnosis. A parameter K defined as K = IRB/Iauto was introduced to reflect the amount of RB in the tissue, where IRB and Iauto represent the fluorescence peak intensity of the RB in the tissue and the autofluorescence intensity of tissue at 580 nm, respectively. The average K values of the four groups were calculated and statistically analyzed by analysis of variance (ANOVA), which revealed statistically significant differences within each group as well as between groups (p < 0.001). After analysis by Clementine 11.1 C&R Tree modeling (CART), the following diagnostic criteria were set: normal, K ≤8.91; simple hyperplasia, 8.91 < K ≤41.92; mild dysplasia, 41.92 < K ≤ 70.79; moderate and severe dysplasia, K >70.79. The sensitivity and specificity to detect precancerous lesions compared with scalpel biopsy were calculated. The results of this study showed that the spectrofluorometric method mediated by RB could accurately discriminate different precancer stages.
AB - A novel in vivo fluorescence spectroscopic diagnostic method has been developed in an animal model to make a quantified precancer diagnosis. In the study, 40 golden hamsters were randomly divided into four groups (groups A, B, C, and D), with group A being the control group and the other three groups being inducted at different precancer stages. A 1% Rose Bengal (RB) solution was used for the fluorescence spectroscopic diagnosis. A parameter K defined as K = IRB/Iauto was introduced to reflect the amount of RB in the tissue, where IRB and Iauto represent the fluorescence peak intensity of the RB in the tissue and the autofluorescence intensity of tissue at 580 nm, respectively. The average K values of the four groups were calculated and statistically analyzed by analysis of variance (ANOVA), which revealed statistically significant differences within each group as well as between groups (p < 0.001). After analysis by Clementine 11.1 C&R Tree modeling (CART), the following diagnostic criteria were set: normal, K ≤8.91; simple hyperplasia, 8.91 < K ≤41.92; mild dysplasia, 41.92 < K ≤ 70.79; moderate and severe dysplasia, K >70.79. The sensitivity and specificity to detect precancerous lesions compared with scalpel biopsy were calculated. The results of this study showed that the spectrofluorometric method mediated by RB could accurately discriminate different precancer stages.
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U2 - 10.1007/s10103-012-1054-y
DO - 10.1007/s10103-012-1054-y
M3 - Article
C2 - 22648285
AN - SCOPUS:84872280146
SN - 0268-8921
VL - 28
SP - 241
EP - 246
JO - Lasers in Medical Science
JF - Lasers in Medical Science
IS - 1
ER -