Abstract
We describe a highly specific RIA for blood and urine 6-keto-PGF1 alpha, the principal metabolite of PGI2. The method requires extraction and column chromatography prior to assay and uses a tritium trace for assay monitoring. Dextran-coated charcoal is used to separate bound from free trace. Acceptable standard curve reproducibility over six assays had a mean correlation of 0.991 with a coefficient of variation of 6.61%. Good precision and accuracy data were generated with this assay, and normal human blood levels in 80 control subjects was less than 125 pg/ml. Significant elevations of blood 6-keto-PGF1 alpha were found in a high proportion of cancer patients with various solid tumor malignancies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 193-199 |
| Number of pages | 7 |
| Journal | Advances in prostaglandin and thromboxane research |
| Volume | 6 |
| State | Published - 1980 |
All Science Journal Classification (ASJC) codes
- General Medicine
