A rapid and sensitive method for the detection of histone peptides

Dennis J. O'Kane; Robert Levenson; Raymond F. Jones

doi:10.1016/0003-2697(78)90336-6

A rapid and sensitive method for the detection of histone peptides

Dennis J. O'Kane, Robert Levenson, Raymond F. Jones

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Fluorescamine has been used to obtain a peptide map of a mixture of histones (H₃, H₂A, H₂B, and H₄) prepared from oocytes of Xenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion of oocyte histones of X. laevis and the peptic digestion of the β chain of insulin. Using the β chain of insulin for a comparison, the 8 major peptide fragments could be separated by electrophoresis within 30 min and were detectable at the picomols level. Some 70 peptide spots of X. laevis oocyte histones were resolved, thus permitting the analysis of this complex mixture of polypeptides without the need for prior separation.

Original language	English (US)
Pages (from-to)	214-219
Number of pages	6
Journal	Analytical Biochemistry
Volume	86
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(78)90336-6
State	Published - May 1978

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(78)90336-6

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@article{c089ca1d9c334f52abfc59c045637898,

title = "A rapid and sensitive method for the detection of histone peptides",

abstract = "Fluorescamine has been used to obtain a peptide map of a mixture of histones (H3, H2A, H2B, and H4) prepared from oocytes of Xenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion of oocyte histones of X. laevis and the peptic digestion of the β chain of insulin. Using the β chain of insulin for a comparison, the 8 major peptide fragments could be separated by electrophoresis within 30 min and were detectable at the picomols level. Some 70 peptide spots of X. laevis oocyte histones were resolved, thus permitting the analysis of this complex mixture of polypeptides without the need for prior separation.",

author = "O'Kane, {Dennis J.} and Robert Levenson and Jones, {Raymond F.}",

note = "Funding Information: This work was supported by Grants BMS73-06899 A01 from the National Science Foundation and HD-07986 from the National Institutes of Health. The authors gratefully acknowledge the help of Mr. John Fermanis of the Department of Microbiology with photography and Dr. Robert W. Merriam of the Department of Cellular and Comparative Biology for his advice and assistance during this study.",

year = "1978",

month = may,

doi = "10.1016/0003-2697(78)90336-6",

language = "English (US)",

volume = "86",

pages = "214--219",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A rapid and sensitive method for the detection of histone peptides

AU - O'Kane, Dennis J.

AU - Levenson, Robert

AU - Jones, Raymond F.

N1 - Funding Information: This work was supported by Grants BMS73-06899 A01 from the National Science Foundation and HD-07986 from the National Institutes of Health. The authors gratefully acknowledge the help of Mr. John Fermanis of the Department of Microbiology with photography and Dr. Robert W. Merriam of the Department of Cellular and Comparative Biology for his advice and assistance during this study.

PY - 1978/5

Y1 - 1978/5

N2 - Fluorescamine has been used to obtain a peptide map of a mixture of histones (H3, H2A, H2B, and H4) prepared from oocytes of Xenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion of oocyte histones of X. laevis and the peptic digestion of the β chain of insulin. Using the β chain of insulin for a comparison, the 8 major peptide fragments could be separated by electrophoresis within 30 min and were detectable at the picomols level. Some 70 peptide spots of X. laevis oocyte histones were resolved, thus permitting the analysis of this complex mixture of polypeptides without the need for prior separation.

AB - Fluorescamine has been used to obtain a peptide map of a mixture of histones (H3, H2A, H2B, and H4) prepared from oocytes of Xenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion of oocyte histones of X. laevis and the peptic digestion of the β chain of insulin. Using the β chain of insulin for a comparison, the 8 major peptide fragments could be separated by electrophoresis within 30 min and were detectable at the picomols level. Some 70 peptide spots of X. laevis oocyte histones were resolved, thus permitting the analysis of this complex mixture of polypeptides without the need for prior separation.

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U2 - 10.1016/0003-2697(78)90336-6

DO - 10.1016/0003-2697(78)90336-6

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AN - SCOPUS:0018128886

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SP - 214

EP - 219

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

A rapid and sensitive method for the detection of histone peptides

Abstract

All Science Journal Classification (ASJC) codes

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