A rapid, automated surface protein profiling of single circulating exosomes in human blood

Golam Kibria; Erika K. Ramos; Katelyn E. Lee; Sarah Bedoyan; Simo Huang; Ravand Samaeekia; Jaffre J. Athman; Clifford V. Harding; Jan Lötvall; Lyndsay Harris; Cheryl L. Thompson; Huiping Liu

doi:10.1038/srep36502

A rapid, automated surface protein profiling of single circulating exosomes in human blood

Golam Kibria, Erika K. Ramos, Katelyn E. Lee, Sarah Bedoyan, Simo Huang, Ravand Samaeekia, Jaffre J. Athman, Clifford V. Harding, Jan Lötvall, Lyndsay Harris, Cheryl L. Thompson, Huiping Liu

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30-150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids.

Original language	English (US)
Article number	36502
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep36502
State	Published - Nov 7 2016

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title = "A rapid, automated surface protein profiling of single circulating exosomes in human blood",

abstract = "Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30-150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids.",

author = "Golam Kibria and Ramos, {Erika K.} and Lee, {Katelyn E.} and Sarah Bedoyan and Simo Huang and Ravand Samaeekia and Athman, {Jaffre J.} and Harding, {Clifford V.} and Jan L{\"o}tvall and Lyndsay Harris and Thompson, {Cheryl L.} and Huiping Liu",

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year = "2016",

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doi = "10.1038/srep36502",

language = "English (US)",

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T1 - A rapid, automated surface protein profiling of single circulating exosomes in human blood

AU - Kibria, Golam

AU - Ramos, Erika K.

AU - Lee, Katelyn E.

AU - Bedoyan, Sarah

AU - Huang, Simo

AU - Samaeekia, Ravand

AU - Athman, Jaffre J.

AU - Harding, Clifford V.

AU - Lötvall, Jan

AU - Harris, Lyndsay

AU - Thompson, Cheryl L.

AU - Liu, Huiping

PY - 2016/11/7

Y1 - 2016/11/7

N2 - Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30-150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids.

AB - Circulating exosomes provide a promising approach to assess novel and dynamic biomarkers in human disease, due to their stability, accessibility and representation of molecules from source cells. However, this potential has been stymied by lack of approaches for molecular profiling of individual exosomes, which have a diameter of 30-150 nm. Here we report a rapid analysis approach to evaluate heterogeneous surface protein expression in single circulating exosomes from human blood. Our studies show a differential CD47 expression in blood-derived individual circulating exosomes that is correlated with breast cancer status, demonstrating a great potential of individual exosome profiles in biomarker discovery. The sensitive and high throughput platform of single exosome analysis can also be applied to characterizing exosomes derived from other patient fluids.

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A rapid, automated surface protein profiling of single circulating exosomes in human blood

Abstract

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