TY - JOUR
T1 - A rapid, sensitive assay for cocaine and its metabolites in biological fluids using solid-phase extraction and high-performance liquid chromatography
AU - Jamdar, Subhash C.
AU - Pantuck, Carol B.
AU - Diaz, Jaime
AU - Mets, Berend
N1 - Funding Information:
The study was supported by the Department of Anesthesiology Staff Research Fund.
PY - 2000
Y1 - 2000
N2 - An improved method for the simultaneous determination of cocaine and its metabolites, benzoylecgonine (BE), norcocaine, and ecgoninemethylester (EME), in rat plasma and urine is described. Following derivatization of EME to p-fluorococaine, chromatography was performed on two high-performance liquid chromatography (HPLC) columns in series (5-μm spheric C8 and 5-μm cyanopropyl) using a mobile phase containing acetonitrile/HPLC water/trifluoroacetic acid (28:72:0.1) with bupivacaine as an internal standard. Quantitation limits were 25 ng/mL for cocaine, BE, and norcocaine and 50 ng/mL for EME using 300-500 μL rat plasma and 500 μL of rat urine. The assay was linear from the limit of quantitation to 2000 ng/mL for cocaine and its metabolites in both plasma and urine samples. Because this method uses a small amount of sample (300 μL plasma or 500 μL of urine), it is applicable to study of the pharmacokinetics and disposition of cocaine and its major metabolites.
AB - An improved method for the simultaneous determination of cocaine and its metabolites, benzoylecgonine (BE), norcocaine, and ecgoninemethylester (EME), in rat plasma and urine is described. Following derivatization of EME to p-fluorococaine, chromatography was performed on two high-performance liquid chromatography (HPLC) columns in series (5-μm spheric C8 and 5-μm cyanopropyl) using a mobile phase containing acetonitrile/HPLC water/trifluoroacetic acid (28:72:0.1) with bupivacaine as an internal standard. Quantitation limits were 25 ng/mL for cocaine, BE, and norcocaine and 50 ng/mL for EME using 300-500 μL rat plasma and 500 μL of rat urine. The assay was linear from the limit of quantitation to 2000 ng/mL for cocaine and its metabolites in both plasma and urine samples. Because this method uses a small amount of sample (300 μL plasma or 500 μL of urine), it is applicable to study of the pharmacokinetics and disposition of cocaine and its major metabolites.
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U2 - 10.1093/jat/24.6.438
DO - 10.1093/jat/24.6.438
M3 - Article
C2 - 10999350
AN - SCOPUS:0033812926
SN - 0146-4760
VL - 24
SP - 438
EP - 441
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
IS - 6
ER -