TY - GEN
T1 - A Reconfigurable Microfluidic Building Block Platform for High-Throughput Nonhormonal Contraceptive Screening
AU - Lee, Jyong Huei
AU - Van Der Linden, Carl
AU - Diaz, Francisco J.
AU - Wong, Pak Kin
N1 - Publisher Copyright:
© 2021 IEEE.
PY - 2021
Y1 - 2021
N2 - This study reports a reconfigurable microfluidic platform comprising multifunctional building blocks and interfaces for high-throughput screening of nonhormonal contraceptives. The platform has three layers of microfluidic building blocks. The top layer consists of interchangeable microchannel building blocks in 3D for liquid handling (e.g., compound libraries, chemical concentration gradients, and drug cocktail mixers). The middle layer incorporates a multiwell array with embedded membrane filters for oocyte culture, medium exchange, enzymatic cumulus cell removal, washing, and fluorescence staining. The bottom layer can be reconfigured for waste collection, oocyte culture, and high-resolution microscopy. The reconfigurability of the platform is enabled by reversible and robust elastomer-based interconnects between building blocks. We demonstrate the platform for performing contraceptive drug loading, in situ oocyte incubation, enzymatic cumulus cell digestion, fluorescence staining, and on-chip imaging in a cumulusoocyte complex (COC) expansion assay for screening nonhormonal contraceptives.
AB - This study reports a reconfigurable microfluidic platform comprising multifunctional building blocks and interfaces for high-throughput screening of nonhormonal contraceptives. The platform has three layers of microfluidic building blocks. The top layer consists of interchangeable microchannel building blocks in 3D for liquid handling (e.g., compound libraries, chemical concentration gradients, and drug cocktail mixers). The middle layer incorporates a multiwell array with embedded membrane filters for oocyte culture, medium exchange, enzymatic cumulus cell removal, washing, and fluorescence staining. The bottom layer can be reconfigured for waste collection, oocyte culture, and high-resolution microscopy. The reconfigurability of the platform is enabled by reversible and robust elastomer-based interconnects between building blocks. We demonstrate the platform for performing contraceptive drug loading, in situ oocyte incubation, enzymatic cumulus cell digestion, fluorescence staining, and on-chip imaging in a cumulusoocyte complex (COC) expansion assay for screening nonhormonal contraceptives.
UR - http://www.scopus.com/inward/record.url?scp=85130321689&partnerID=8YFLogxK
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U2 - 10.1109/NANOMED54179.2021.9766590
DO - 10.1109/NANOMED54179.2021.9766590
M3 - Conference contribution
AN - SCOPUS:85130321689
T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
SP - 72
EP - 75
BT - Proceedings of the 15th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2021
PB - IEEE Computer Society
T2 - 15th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2021
Y2 - 15 November 2021 through 17 November 2021
ER -