Abstract
A 73-kilodalton (kD) intracellular protein was found to bind to peptide regions that target intracellular proteins for lysosomal degradation in response to serum withdrawal. This protein cross-reacted with a monoclonal antibody raised to a member of the 70-kD heat shock protein (hsp70) family, and sequences of two internal peptides of the 73-kD protein confirm that it is a member of this family. In response to serum withdrawal, the intracellular concentration of the 73-kD protein increased severalfold. In the presence of adenosine 5′-triphosphate (ATP) and MgCl2, the 73-kD protein enhanced protein degradation in two different cell-free assays for lysosomal proteolysis.
Original language | English (US) |
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Pages (from-to) | 382-385 |
Number of pages | 4 |
Journal | Science |
Volume | 246 |
Issue number | 4928 |
DOIs | |
State | Published - 1989 |
All Science Journal Classification (ASJC) codes
- General