TY - JOUR
T1 - A serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis
AU - Orth, A. B.
AU - Rzhetskaya, M.
AU - Pell, E. J.
AU - Tien, M.
PY - 1995
Y1 - 1995
N2 - A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance- conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.
AB - A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance- conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.
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U2 - 10.1128/aem.61.6.2341-2345.1995
DO - 10.1128/aem.61.6.2341-2345.1995
M3 - Article
C2 - 7793954
AN - SCOPUS:0029047718
SN - 0099-2240
VL - 61
SP - 2341
EP - 2345
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 6
ER -