Abstract
A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance- conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2341-2345 |
| Number of pages | 5 |
| Journal | Applied and environmental microbiology |
| Volume | 61 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1995 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
- Ecology
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