A simple, nonenzymatic method for desialylating polysialylated ganglio- N-tetraose series gangliosides to produce GM1

Cara Lynne Schengrund; Pavol Kováč

doi:10.1016/s0022-2275(20)33351-4

A simple, nonenzymatic method for desialylating polysialylated ganglio- N-tetraose series gangliosides to produce GM1

Cara Lynne Schengrund, Pavol Kováč

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5 Scopus citations

Abstract

Dowex-50W-H⁺ was used to catalyze the highly selective desialylation of polysialylated ganglio-N-tetraose series gangliosides to yield primarily GM1. High performance thin-layer chromatographic analysis of recovered lipid indicated that 60-70% of the recovered ganglioside was GM1. Identification of the major product as GM1 was confirmed by proton NMR spectra and lack of sialic acid release by Vibrio cholerae sialidase.

Original language	English (US)
Pages (from-to)	160-163
Number of pages	4
Journal	Journal of Lipid Research
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/s0022-2275(20)33351-4
State	Published - Jan 1999

All Science Journal Classification (ASJC) codes

Biochemistry
Endocrinology
Cell Biology

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10.1016/s0022-2275(20)33351-4

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AB - Dowex-50W-H+ was used to catalyze the highly selective desialylation of polysialylated ganglio-N-tetraose series gangliosides to yield primarily GM1. High performance thin-layer chromatographic analysis of recovered lipid indicated that 60-70% of the recovered ganglioside was GM1. Identification of the major product as GM1 was confirmed by proton NMR spectra and lack of sialic acid release by Vibrio cholerae sialidase.

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A simple, nonenzymatic method for desialylating polysialylated ganglio- N-tetraose series gangliosides to produce GM1

Abstract

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