Abstract
The Multiskan spectrophotometric system and Coomassie brilliant blue G-250 protein-dye binding method have been used together to measure NaOH-solubilized protein in subcellular membrane fractions prepared from isolated rat adipose cells. Forty-eight samples can be read in duplicate within 1 min. Sucrose in concentrations up to 0.7 m interfere only moderately with the assay. A rapid and convenient method is, therefore, now available for multiple protein determinations following sucellular fractionation on sucrose gradients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 424-427 |
| Number of pages | 4 |
| Journal | Analytical Biochemistry |
| Volume | 119 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 15 1982 |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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