TY - GEN
T1 - A single molecule barcode nanobiosensor for dynamic multigene analysis in live cells during tissue morphogenesis and wound healing
AU - Lu, Yi
AU - Wong, Pak Kin
N1 - Funding Information:
This project is supported by the NSF Biophotonic program.
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - This study reports a single molecule barcode nanobiosensor for multigene expression analysis at the single cell level. Using a multispectral single molecule sensing (MSMS) system, the dual-labeled barcode probes can be resolved for single molecule tracking, super-resolution localization, multispectral imaging, and spectral unmixing. We demonstrate multiplex detection at the single molecule level for investigating the interplays between Notch1-Dll4 and Nrf2 signaling in leader cell formation during collective cell migration.
AB - This study reports a single molecule barcode nanobiosensor for multigene expression analysis at the single cell level. Using a multispectral single molecule sensing (MSMS) system, the dual-labeled barcode probes can be resolved for single molecule tracking, super-resolution localization, multispectral imaging, and spectral unmixing. We demonstrate multiplex detection at the single molecule level for investigating the interplays between Notch1-Dll4 and Nrf2 signaling in leader cell formation during collective cell migration.
UR - http://www.scopus.com/inward/record.url?scp=85094947503&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85094947503&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85094947503
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 338
EP - 339
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -